Citation: ZHANG Yan-peng, LI Jing, KOU Zheng, CHEN Sheng-liang, FAN Zhao-jun, ZHANG Zhong, LI Tian-xian*. Isolation and Identification of Duck Parvovirus 04Nb Strain and Sequence Analysis of rep Gene .VIROLOGICA SINICA, 2006, 21(2) : 173-177.

Isolation and Identification of Duck Parvovirus 04Nb Strain and Sequence Analysis of rep Gene

  • Available online: 20 March 2006
  • A severe contagious disease broke out in several duckeries in Ningbo, Zhejiang province with the symptoms of diarrhea and grasping. The liver tissues from the dead ducks were collected and inoculated into the allantoic cavity of the 11-day-old embryos. Through purification by sucrose density gradient centrifugation, a diameter of 20nm virions with typical duck parvovirus (DPV) characters was observed by electron microscopy. In the test of agar-gel immunodiffusion, the reaction between the stuff from dead embryos and positive serum was obvious. Through SDS-PAGE, three main segments were visible which coincided with DPV. In animal test, similar symptoms were also observed. Using the primers designed according to DPV genome, we acquired an anticipative 600bp DNA fragment, which was cloned into E.coli and sequenced. The sequence shared 98% identity with the representative DPV strain. From all above results, the pathologic agent was diagnosed as Duck Parvovirus. To acquire more information of the rep gene, we sequenced and aligned it with 4 other sequences of DPV or goose parvovirus (GPV) from GenBank. The result indicated the homology of rep gene was about 98% with DPV and 81% with GPV.

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    Isolation and Identification of Duck Parvovirus 04Nb Strain and Sequence Analysis of rep Gene

    • 1. The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    Abstract: A severe contagious disease broke out in several duckeries in Ningbo, Zhejiang province with the symptoms of diarrhea and grasping. The liver tissues from the dead ducks were collected and inoculated into the allantoic cavity of the 11-day-old embryos. Through purification by sucrose density gradient centrifugation, a diameter of 20nm virions with typical duck parvovirus (DPV) characters was observed by electron microscopy. In the test of agar-gel immunodiffusion, the reaction between the stuff from dead embryos and positive serum was obvious. Through SDS-PAGE, three main segments were visible which coincided with DPV. In animal test, similar symptoms were also observed. Using the primers designed according to DPV genome, we acquired an anticipative 600bp DNA fragment, which was cloned into E.coli and sequenced. The sequence shared 98% identity with the representative DPV strain. From all above results, the pathologic agent was diagnosed as Duck Parvovirus. To acquire more information of the rep gene, we sequenced and aligned it with 4 other sequences of DPV or goose parvovirus (GPV) from GenBank. The result indicated the homology of rep gene was about 98% with DPV and 81% with GPV.

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