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Citation: WEI Li-li, WANG Xiao-jun, GENG Qing-hua, TONG Xiao, LIANG Hua, SHEN Tao, ZHANG Xiao-yan, XIANG Wen-hua, SHAO Yi-ming, SHEN Rong-xian. Characterization of a LTR Chimeric Infectious Clone of Chinese Equine Infectious Anemia Virus [J].VIROLOGICA SINICA, 2006, 21(3) : 244-248.

Characterization of a LTR Chimeric Infectious Clone of Chinese Equine Infectious Anemia Virus

  • Corresponding author: SHEN Rong-xian, 
  • Equine infectious anemia virus full-length-gene chimeric clone derived virus, vLGFD9-12, was inoculated into healthy horses. Over an observation period of 150 days, all inoculated groups showed no overt abnormal symptoms. The results from routine blood assays showed that the counts of WBC and HGB had no obvious changes between vLGFD9-12 group and its parental virus pLGFD3-8. Low levels of viral RNA in the plasma of vLGFD9-12- and pLGFD3-8-infected horses was detected, The results from cytolytic T lymphocyte (CTL) and lymphocyte proliferation assay (LPA) showed that all inoculated animals had similar changes on virus-specific CTL responses and virus-specific lymphocyte proliferation activity. These results provide an important foundation for further study on mechanism of attenuation of the EIAV and the protective immunity against EIAV.

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    Characterization of a LTR Chimeric Infectious Clone of Chinese Equine Infectious Anemia Virus

      Corresponding author: SHEN Rong-xian,
    • 1. 1. Harbin Veterinary Research Institute of CAAS, Harbin 150001
    • 2. China Institute of Veterinary Drug Control, Beijing 150081
    • 3. National Center for AIDS/STD Prevention and Control, China CDC, Beijing 100050

    Abstract: Equine infectious anemia virus full-length-gene chimeric clone derived virus, vLGFD9-12, was inoculated into healthy horses. Over an observation period of 150 days, all inoculated groups showed no overt abnormal symptoms. The results from routine blood assays showed that the counts of WBC and HGB had no obvious changes between vLGFD9-12 group and its parental virus pLGFD3-8. Low levels of viral RNA in the plasma of vLGFD9-12- and pLGFD3-8-infected horses was detected, The results from cytolytic T lymphocyte (CTL) and lymphocyte proliferation assay (LPA) showed that all inoculated animals had similar changes on virus-specific CTL responses and virus-specific lymphocyte proliferation activity. These results provide an important foundation for further study on mechanism of attenuation of the EIAV and the protective immunity against EIAV.

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