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Citation: XIE Zhi-xun, TANG Xiao-fei, DONG Jian-bao, LIU Jia-bo, PANG Yao-shan, DENG Xian-wen, XIE Zhi-qin. Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi [J].VIROLOGICA SINICA, 2006, 21(3) : 261-266.

Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi

  • Corresponding author: XIE Zhi-xun, 
  • Eight pairs of specific primers were designed and synthesized based on the entire genomic sequence data of Newcastle Disease Virus (NDV) present in GenBank. Complete nucleotide sequences of GX7/02, GX9/03 and GX11/03 strains were acquired by reverse transcription-polymerase reaction. The entire genomic sequences of three NDV strains consisted of 15 192 nt, which was the same in length to 7 NDV ZJ1, U.S/Largo/71, Italy/2736/00 strains, but was 6 nt longer than LaSota, Clone-30 and B1 strains in GenBank. The additional 6 nt motif was located in the non-coding region of nucleoprotein (NP) gene between nucleotides 1647 and 1648 of the entire genomic sequences of NDV LaSota, B1 and Clone-30 strains. The results of identity analysis of nucleotide and amino acid sequences showed that the identities between GX7/02, GX9/03, GX11/03 and ZJ1 strains were higher than that of the three strains of LaSota, B1 and Clone-30.

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    Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi

      Corresponding author: XIE Zhi-xun,
    • 1. 1.Guangxi Veterinary Research Institute, Nanning, 530001, China
    • 2. College of Animal Sciences and Technology, Guangxi University, Nanning, 530005, China

    Abstract: Eight pairs of specific primers were designed and synthesized based on the entire genomic sequence data of Newcastle Disease Virus (NDV) present in GenBank. Complete nucleotide sequences of GX7/02, GX9/03 and GX11/03 strains were acquired by reverse transcription-polymerase reaction. The entire genomic sequences of three NDV strains consisted of 15 192 nt, which was the same in length to 7 NDV ZJ1, U.S/Largo/71, Italy/2736/00 strains, but was 6 nt longer than LaSota, Clone-30 and B1 strains in GenBank. The additional 6 nt motif was located in the non-coding region of nucleoprotein (NP) gene between nucleotides 1647 and 1648 of the entire genomic sequences of NDV LaSota, B1 and Clone-30 strains. The results of identity analysis of nucleotide and amino acid sequences showed that the identities between GX7/02, GX9/03, GX11/03 and ZJ1 strains were higher than that of the three strains of LaSota, B1 and Clone-30.

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