Anti-CVB3 Polypeptides Selected from A Phage Display Random Peptide Library

CVB3 was propagated in Hep-2 cells and purified by sucrose gradient centrifugation. The purified CVB3 reacted with random peptides library displaying 9 amino acids. After 3 rounds of screening, the capability of phage peptides in the inhibition of CVB3 replication was determined. DNA was extracted from the positive phage clones, sequenced and the amion acid sequence was deduced. The results showed that 3 phage positive peptides were identified and bound to CVB3 with high affinity. The binding of the peptide to CVB3 was shown to inhibit replication substantially. The TCID50 of progeny virus was reduced from 10-7.5SFU/mL to 10-5.25, 10-6, 10-5.5SFU/mL by the three peptides, respectively. The data demonstrate that peptides against CVB3 can be selected from phage peptide library and may prove useful as antiviral peptides agents.