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Citation: LIU Ming-gui, WANG Ye-fu, SUI Fang, WAN Zhi-xiang, GAO Wen-jun. A Visual Gene Array for the Detection of HSV-2 Based on Nanogold Probes [J].VIROLOGICA SINICA, 2006, 21(3) : 294-297.

A Visual Gene Array for the Detection of HSV-2 Based on Nanogold Probes

  • Corresponding author: WANG Ye-fu, 
  • A visual HSV-2 detection gene array was developed based on nanogold probes。The specific primer and probe of HSV-2 were designed based on the highly conservative region of HSV-2 DNA polymerase gene and the HSV-2 PCR amplicon were labeled by biotin through PCR reaction.Amino-modified probes were spotted on an activated glass surface on which hybridized with the PCR amplicon.Subsequently, the streptavidin-nanogold was added to form the amplification system of the biotin-streptavidin-nanogold complex.At last, the step of silver staining amplified the signal of hybridization, Therefore it was easy to detect the HSV-2 by naked eyes.As little as 100fmol/L of HSV-2 amplicon could be detected on the array, The HSV-2 detection gene array can detect the HSV-2 virus infection with high sensitivity and low cost.

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    A Visual Gene Array for the Detection of HSV-2 Based on Nanogold Probes

      Corresponding author: WANG Ye-fu,
    • 1. 1. State key Lab of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
    • 2. Liaoyang Center Hospital, Liaoyang 111000, China

    Abstract: A visual HSV-2 detection gene array was developed based on nanogold probes。The specific primer and probe of HSV-2 were designed based on the highly conservative region of HSV-2 DNA polymerase gene and the HSV-2 PCR amplicon were labeled by biotin through PCR reaction.Amino-modified probes were spotted on an activated glass surface on which hybridized with the PCR amplicon.Subsequently, the streptavidin-nanogold was added to form the amplification system of the biotin-streptavidin-nanogold complex.At last, the step of silver staining amplified the signal of hybridization, Therefore it was easy to detect the HSV-2 by naked eyes.As little as 100fmol/L of HSV-2 amplicon could be detected on the array, The HSV-2 detection gene array can detect the HSV-2 virus infection with high sensitivity and low cost.

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