Construction and Application of Eukaryotic Expression Vector phIE Containing HSVI α-gene Promoter
Abstract: An eukaryotic expression vector phIE composed of pcDNA3 skeleton and the herpes simplex virus immediate early gene promoter was constructed to provide a new choice for ectopic expression. The plasmid phIE-Us1 was constructed by inserting the us1 cDNA downstream of the promoter to analyze the function of ICP22 protein very early in the infection process. Furthermore, the gene encoding the chloramphenicol acetyl-transferase was inserted into the multiple cloning site of the expression vector to yield the phIE-CAT. In functional analysis of viral protein ICP22 and Vp16, phIE-CAT was confirmed to be capable of evaluating the mechanism of the genome of herpes simplex virus 1 transcriptional regulation.