Construction of Recombinant Adenovirus Containing Pocine IFN-α genes and Activity of Anti-Swine Foot- and-mouth Disease Virus

Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction (RT-PCR), and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages. This recombinant adenovirus rAd-poIFN-α could be stably passaged in HEK-293A cells and generated titres in the order of 107 TCID50/mL. The mRNA of the transgene in this recombinant adenovirus was detected by RT-PCR. As a result, the adenovirus exhibited high anti-swine FMDV activity in PK-15 cells and could be further studied in the control strategy of swine FMDV.