Citation: ZHANG Wan-ju, ZHAO Shu-ling, ZHANG Xiao-xia, TANG Xian-chun, XIAO Yu-zhou, MEI Chun-lei, SUN Xiu-lian, PENG Hui-ying. Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus .VIROLOGICA SINICA, 2006, 21(4) : 401-404.

Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus

  • Available online: 20 July 2006
  • The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows the detection of protein-RNA interactions in the test tube, the possible interactions were tested by the recombinant protein and the double-strand RNA (the genome of DpCPV).

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    Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus

    • 1. 1.Virus Molecular Ecology Lab, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
    • 2. The Graduate School of Chinese Academy of Sciences, Beijing 100039, China

    Abstract: The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows the detection of protein-RNA interactions in the test tube, the possible interactions were tested by the recombinant protein and the double-strand RNA (the genome of DpCPV).

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