Expression and Functional Characterization of NS5 of Dendrolimus punctatus Cypovirus
Abstract: The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows the detection of protein-RNA interactions in the test tube, the possible interactions were tested by the recombinant protein and the double-strand RNA (the genome of DpCPV).