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Volume 21 Issue 5
October 2006
    Citation: GONG Zhen-li, LIU Xiang-tao, TIAN Hong, WU Jin-yan, DAI Xing-guo, SHANG You-jun, CHEN Guo-dong. Expression of FMDV P1+2A Gene in BHK-21 Cells .VIROLOGICA SINICA, 2006, 21(5) : 454-457.
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    Expression of FMDV P1+2A Gene in BHK-21 Cells

    • 1.

      Key Laboratory of Animal Virology, Ministry of Agriculture Lanzhou Veterinary Research Institute, Lanzhou 730046,China

    • Available online: 20 September 2006
    • In this study, recombinant expression plasmid pQE-Tri/P1+2A was constructed and then transfected into BHK-21 cell line mediated by liposome 2000. The results of SDS-PAGE、Western-blotting、fluorescence antibody and ELISA showed that FMDV P1+2A protein was expressed in BHK-21 cell line successfully and can be recognized by FMD positive serum. The result will lay a foundation for study of FMDV gene engineered vaccines as well as the development of diagnostic reagents.
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      Expression of FMDV P1+2A Gene in BHK-21 Cells

      • 1. Key Laboratory of Animal Virology, Ministry of Agriculture Lanzhou Veterinary Research Institute, Lanzhou 730046,China

      Abstract: In this study, recombinant expression plasmid pQE-Tri/P1+2A was constructed and then transfected into BHK-21 cell line mediated by liposome 2000. The results of SDS-PAGE、Western-blotting、fluorescence antibody and ELISA showed that FMDV P1+2A protein was expressed in BHK-21 cell line successfully and can be recognized by FMD positive serum. The result will lay a foundation for study of FMDV gene engineered vaccines as well as the development of diagnostic reagents.

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