Citation: DONG Chun-sheng, DENG Fei, YUAN Li, PAN Xiao-yu, WANG Hua-lin, HU Zhi-hong. Deletion of p10 From the Genome of HaSNPV Results in a Faulty Polyhedral Envelope .VIROLOGICA SINICA, 2006, 21(6) : 594-598.

Deletion of p10 From the Genome of HaSNPV Results in a Faulty Polyhedral Envelope

  • Corresponding author: HU Zhi-hong, 
  • Available online: 20 November 2006
  • p10 is one of the two very late genes that are highly expressed in baculovirus infected cells. We have studied the function of P10 by constructing a p10 deletion recombinant of the Helicoverpa armigera singly-enveloped nucleopolyhedrovirus (HaSNPV). The p10 gene of HaSNPV bacterial artificial chromosome (HaBacHZ8) was replaced with a DNA fragment containing a chloramphenicol antibiotic gene and a 40bp flanking region using the λ phage Red recombination system in E. coli BW25113. Polyhedrin and gfp genes were then translocated to the p10 deleted HaBacHZ8 using a Bac-to-Bac system generating the recombinant bacmid HaBacΔp10-PH-eGFP. Virus growth curves and bioassays indicated that deletion of p10 did not impair the infectivity of virus in vitro and in vivo. However, electronic microscopic analysis revealed that deletion of P10 resulted in the fragmentation of polyhedral envelope.

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    Deletion of p10 From the Genome of HaSNPV Results in a Faulty Polyhedral Envelope

      Corresponding author: HU Zhi-hong,
    • 1. 1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
    • 2. Graduate School of Chinese Academy of Science, Beijing 100039, China

    Abstract: p10 is one of the two very late genes that are highly expressed in baculovirus infected cells. We have studied the function of P10 by constructing a p10 deletion recombinant of the Helicoverpa armigera singly-enveloped nucleopolyhedrovirus (HaSNPV). The p10 gene of HaSNPV bacterial artificial chromosome (HaBacHZ8) was replaced with a DNA fragment containing a chloramphenicol antibiotic gene and a 40bp flanking region using the λ phage Red recombination system in E. coli BW25113. Polyhedrin and gfp genes were then translocated to the p10 deleted HaBacHZ8 using a Bac-to-Bac system generating the recombinant bacmid HaBacΔp10-PH-eGFP. Virus growth curves and bioassays indicated that deletion of p10 did not impair the infectivity of virus in vitro and in vivo. However, electronic microscopic analysis revealed that deletion of P10 resulted in the fragmentation of polyhedral envelope.

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