The infection kinetics and transmission potential of two Guaico Culex viruses in <em>Culex quinquefasciatus</em> mosquitoes

Ru-Yi Chen; Teng Zhao; Jing-Jing Guo; Feng Zhu; Na-Na Zhang; Xiao-Feng Li; Hai-Tao Liu; Fei Wang; Yong-Qiang Deng; Cheng-Feng Qin

doi:10.1016/j.virs.2024.03.002

April 2024

Citation: Ru-Yi Chen, Teng Zhao, Jing-Jing Guo, Feng Zhu, Na-Na Zhang, Xiao-Feng Li, Hai-Tao Liu, Fei Wang, Yong-Qiang Deng, Cheng-Feng Qin. The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes .VIROLOGICA SINICA, 2024, 39(2) : 228-234. http://dx.doi.org/10.1016/j.virs.2024.03.002

The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes

Ru-Yi Chen ^a,b ,
Teng Zhao ^b ,
Jing-Jing Guo ^b ,
Feng Zhu ^b,c ,
Na-Na Zhang ^b ,
Xiao-Feng Li ^b ,
Hai-Tao Liu ^b ,
Fei Wang ^{a
,,} ,
Yong-Qiang Deng ^{b
,,} ,
Cheng-Feng Qin ^{b
,,}

a. College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China;
b. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China;
c. School of Life Sciences, Southwest Forestry University, Kunming, 650224, China

Corresponding author: Fei Wang, wangfei179@163.com
Yong-Qiang Deng, dengyq1977@126.com
Cheng-Feng Qin, qincf@bmi.ac.cn
Received Date: 26 August 2023
Accepted Date: 05 March 2024
Available online: 08 March 2024

Abstract

Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 cells. Further in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of Culex quinquefasciatus at 4–10 days after oral infection. In addition, two GCXVs can colonize Cx. quinquefasciatus eggs, resulting in positive rates of 15%–35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.
- Guaico Culex virus (GCXV)
- , Jingmenvirus
- , Culex quinquefasciatus
- , Vertical transmission

Electronic Supplementary Material

10.1016j.virs.2024.03.002-ESM.docx
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