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Volume 39 Issue 2
April 2024

    . doi: 10.1016/j.virs.2024.03.002
    Citation: Ru-Yi Chen, Teng Zhao, Jing-Jing Guo, Feng Zhu, Na-Na Zhang, Xiao-Feng Li, Hai-Tao Liu, Fei Wang, Yong-Qiang Deng, Cheng-Feng Qin. The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes .VIROLOGICA SINICA, 2024, 39(2) : 228-234.  http://dx.doi.org/10.1016/j.virs.2024.03.002
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    两种瓜伊科库蚊病毒对致倦库蚊的感染动力学及传播潜力

    • a. 江西农业大学生物科学与生物工程学院, 南昌 330045;

    • b. 病原微生物生物安全全国重点实验室, 军事医学研究院微生物与流行病学研究所, 北京 100071;

    • c. 西南林业大学生命科学学院, 昆明 650224

    • 瓜伊库蚊病毒（GCXV）是一种新分离鉴定的从中美洲和南美洲库蚊分离获得的分节段病毒，其基因组由4到5个单股正链RNA片段组成。目前，GCXV在蚊子体内的感染动力学和传播能力尚不清楚。在本研究中，我们首先使用反向遗传学方法在C6/36细胞中拯救了分别包含4个和5个RNA片段的两种GCXVs（4S和5S）。体外生物学鉴定进一步表明，两种GCXV表现出相当的复制动力学、蛋白表达能力和病毒滴度。重要的是，将两种GCXV经口感染致倦库蚊，在感染后4-10天的身体、唾液腺、中肠和卵巢中均检测到GCXV特异的RNA。此外，两种GCXV可定植于蚊卵，导致第二个生殖腺营养周期的阳性率为15%-35%。综上所述，我们的研究结果表明，在经口感染后的第一和第二性腺营养周期的致倦库蚊卵中，均可检测到4或5个RNA片段的GCXVs。

    The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes

    • a. College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China;

    • b. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China;

    • c. School of Life Sciences, Southwest Forestry University, Kunming, 650224, China

    • Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 cells. Further in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of Culex quinquefasciatus at 4–10 days after oral infection. In addition, two GCXVs can colonize Cx. quinquefasciatus eggs, resulting in positive rates of 15%–35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.
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      The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes

        Corresponding author: Fei Wang, wangfei179@163.com
        Corresponding author: Yong-Qiang Deng, dengyq1977@126.com
        Corresponding author: Cheng-Feng Qin, qincf@bmi.ac.cn
      • a. College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China;
      • b. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China;
      • c. School of Life Sciences, Southwest Forestry University, Kunming, 650224, China
      • Received Date: 26 August 2023
      • Accepted Date: 05 March 2024

      Abstract: Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 cells. Further in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of Culex quinquefasciatus at 4–10 days after oral infection. In addition, two GCXVs can colonize Cx. quinquefasciatus eggs, resulting in positive rates of 15%–35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.

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