Serving the Society Since 1986
Advanced Search
Volume 23 Issue 5
October 2008
    Citation: Yu-chen XIA, Zhi-hong HU, Zhi-juan QIU, Zhong-bin MA, Hua-lin WANG, Fei DENG. An Improved Culture System for Virus Isolation and Detection* .VIROLOGICA SINICA, 2008, 23(5) : 345-351.  http://dx.doi.org/10.1007/s12250-008-2968-1
    shu

    An Improved Culture System for Virus Isolation and Detection*

    cstr: 32224.14.s12250-008-2968-1

    • State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • Corresponding author: Fei DENG, df@wh.iov.cn
    • Received Date: 14 April 2008
      Accepted Date: 25 July 2008
      Available online: 01 October 2008

      Fund Project: EPISARS SP22-CT-2004-511603The Knowledge Innovation Program of Chinese Academy of Sciences KSCX2-YW-N-065FP6 projects DISSECT SP22-CT-2004-511060

    • Abstract: Cell culture has played an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology study of viruses. In the present study, a new system that could support multiple different cell lines to be simultaneous cultured in one dish was developed. In the system, each cell line was cultured in an isolated zone in the same dish or well and the system is therefore called isolated co-cultured system. The usefulness of this novel approach for virus isolation was demonstrated using a model system based on adenovirus.
    • 加载中

      1. Allard A, Girones R, Juto P, et al. 1990. Polymerase chain reaction for detection of adenoviruses in stool samples. J Clin Microbiol, 28: 2659-2667.

      2. Brewer L A, Lwamba H C, Murtaugh M P, et al. 2001. Porcine encephalomyocarditis virus persists in pig myocardium and infects human myocardial cells. J Virol, 75: 11621-11629.
          doi: 10.1128/JVI.75.23.11621-11629.2001

      3. Brumback B G, Wade C D. 1994. Simultaneous culture for adenovirus, cytomegalovirus, and herpes simplex virus in same shell vial by using three-color fluorescence. J Clin Microbiol, 32: 2289-2290.

      4. Diane S L, Christine C G. 2007. Role of Cell Culture for Virus Detection in the Age of Technology. Clin Microbiol Rev, 20: 49-78.
          doi: 10.1128/CMR.00002-06

      5. Fedorko D P, Nelson N A, McAuliffe J M, et al. 2006. Performance of the rapid tests for detection of avian influenza A virus types H5N1 and H9N2. J Clin Microbiol, 44: 1596-1597.
          doi: 10.1128/JCM.44.4.1596-1597.2006

      6. Gillim R L, Taylor J, Scholl D R, et al. 2004. Discovery of novel human and animal cells infectedby the severe acute respiratory syndrome coronavirus by replication specific multiplex reverse transcription-PCR. J Clin Microbiol, 42: 3196-3206.
          doi: 10.1128/JCM.42.7.3196-3206.2004

      7. Harit A K, Ichhpujani R L, Gupta S, et al. 2006. Nipah/Hendra virus outbreak in Siliguri, West Bengal, India in 2001. Indian J Med Res, 123: 553-560.

      8. Harrison R. 1907. Observations on the living developing nerve fiber. Anat Rec, 1: 116-128.
          doi: 10.1002/(ISSN)1097-0185

      9. Hsiung G D. 1984. Diagnostic virology: from animals to automation. Yale J Biol Med, 57: 727-733.

      10. Kaye M, Druce J, Tran T, et al. 2006. SARS-associated coronavirus replication in cell lines. Emerg Infect Dis, 12: 128-133.
          doi: 10.3201/eid1201.050496

      11. Ksiazek T G, Erdman D, Goldsmith C S, et al. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med, 348: 1953-1966.
          doi: 10.1056/NEJMoa030781

      12. Li L, Wo J, Shao J, et al. 2003. SARS-coronavirus replicates in mononuclear cells of peripheral blood (PBMCs) from SARS patients. J Clin Virol, 28: 239-244.
          doi: 10.1016/S1386-6532(03)00195-1

      13. Mossel E C, Huang C, Narayanan K, et al. 2005. Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication. J Virol, 79: 3846-3850.
          doi: 10.1128/JVI.79.6.3846-3850.2005

      14. Peiris J S, Chu C M, Cheng V C, et al. 2003. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet, 361: 1319-1325.
          doi: 10.1016/S0140-6736(03)13077-2

      15. Ran A M, Hartwig N G, Bestebroer T M, et al. 2004. A previously undescribed coronavirus associated with respiratory disease in humans. Proc. Natl. Acad. Sci, 101: 6212-6216.
          doi: 10.1073/pnas.0400762101

      16. Ray C G, Minnich L L. 1987. Efficiency of immuno-fluorescence for rapid detection of common respiratory viruses. J Clin Microbiol, 25: 355-357.

      17. Schildgen O, Jebbink M F, Vries M, et al. 2006. Identification of cell lines permissive for human coro-navirus NL63. J Virol Methods, 138: 207-210.
          doi: 10.1016/j.jviromet.2006.07.023

      18. Wen H L, Moore M J, Vasilieva N, et al. 2003. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426: 450-454.
          doi: 10.1038/nature02145

      19. Yuen K Y, Chang P K, Peiris J S, et al. 1998. Clinical features and rapidviral diagnosis of human disease asso-ciated with avian influenza A H5N1 virus. Lancet, 351: 467-471.
          doi: 10.1016/S0140-6736(98)01182-9

    • 加载中

    Figures(4)

    PDF

    Article Metrics

    Article views(6139) PDF downloads(16) Cited by()

    Related
    Proportional views

      An Improved Culture System for Virus Isolation and Detection*

        Corresponding author: Fei DENG, df@wh.iov.cn
      • State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • Received Date: 14 April 2008
      • Accepted Date: 25 July 2008
      Fund Project:  EPISARS SP22-CT-2004-511603The Knowledge Innovation Program of Chinese Academy of Sciences KSCX2-YW-N-065FP6 projects DISSECT SP22-CT-2004-511060

      Abstract: Abstract: Cell culture has played an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology study of viruses. In the present study, a new system that could support multiple different cell lines to be simultaneous cultured in one dish was developed. In the system, each cell line was cultured in an isolated zone in the same dish or well and the system is therefore called isolated co-cultured system. The usefulness of this novel approach for virus isolation was demonstrated using a model system based on adenovirus.

        HTML