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Volume 24 Issue 6
December 2009
    Citation: Shuai SONG, Tong LIN, Jun-jun SHAO, Shan-dian GAO, Guo-zheng CONG, Jun-zheng DU, Hui-yun CHANG. Preparation and Characterization of Monoclonal Antibodies against VP1 Protein of Foot-and-mouth Disease Virus O/China99 .VIROLOGICA SINICA, 2009, 24(6) : 566-572.  http://dx.doi.org/10.1007/s12250-009-3061-0
    shu

    Preparation and Characterization of Monoclonal Antibodies against VP1 Protein of Foot-and-mouth Disease Virus O/China99

    cstr: 32224.14.s12250-009-3061-0
    • 1.

      National FMD Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricutral Science, Lanzhou 730046, China

    • 2.

      Guangdong Open Laboratory of Veterinary Public Healty, Institute of Veterinary Medicine, Guangdong Academy of Agricultural Science, Guangzhou 510640, China

    • Corresponding author: Hui-yun CHANG, changhuiyun@126.com
    • Received Date: 08 April 2009
      Accepted Date: 15 September 2009
      Available online: 01 December 2009
    • Monoclonal antibodies (McAbs) 1A9 and 9F12 against Foot-and-mouth disease virus (FMDV) serotype O were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with O/China99. Both McAbs reacted with O/China99 but not with Asia 1, as determined by immunohistochemistry assay. The microneutralization titer of the McAbs 1A9 and 9F12 were 640 and 1 280, respectively. Both McAbs contain kappa light chains, but the McAbs 1A9 and 9F12 were IgG1 and IgM, respectively. In order to define the McAbs binding epitopes, the reactivity of these McAbs against VP1, P20 and P14 were examined using indirect ELISA, the result showed that both McAbs reacted with VP1 and P20. McAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV.
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      3. Domingo E, Escarmis C, Baranowski E, et al. 2003. Evolution of foot-and-mouth disease virus. Virus Res, 91 (1): 47-63.
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      4. Harlow E, Lane D. 1988. Monoclonal antibodies. In: Antibodies a Laboratory Manual, New York:Cold Spring Harbor Laboratory Press, p139-244.

      5. Hong T H, Chen S T, Tang T K, et al. 1989. The production of polyclonal and monoclonal antibodies in mice using novel immunization methods. J Immunol Methods, 120: 151-157.
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      6. Jackson T, Sharma A, Ghazaleh R A, et al. 1997. Arginine-glycine-aspartic acid-specific binding by foot-and-mouth disease viruses to the purified integrin alpha (v) beta3 in vitro. J Virol, 71: 8357-8361.

      7. Knowles N J, Samuel A R. 2003. Molecular epidemiology of foot-and-mouth disease virus. Virus Res, 91: 65-80.
          doi: 10.1016/S0168-1702(02)00260-5

      8. Mateu M G, Andreu D, Domingo E. 1995. Antibodies raised in a natural host and monoclonal antibodies recognize similar antigenic features of foot-and-mouth disease virus. Virology, 210: 113-124.

      9. Ming Y, Alfonso C, Rachel S B, et al. 2007. Production and characterization of two serotype independent monoclonal antibodies against foot-and-mouth disease virus. Vet Immunol Immunopathol, 115: 126-134.
          doi: 10.1016/j.vetimm.2006.10.002

      10. OIE (World Organization for Animal Health), 2004. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 5th ed, Paris. World Organization for Animal Health, p 111-128.

      11. Reed L J, Muench H. 1938. A simple method of estimating fifty per cent end-points. Am J Hyg, 27: 493-497.

      12. Tesar M, Berger H G, Marquardt O. 1989. Serological probes for some foot-and-mouth disease virus nonstructural proteins. Virus Genes, 3: 29-44.
          doi: 10.1007/BF00301985

      13. Wang J H, Liang C M, Peng J M, et al. 2003. Induction of immunity in swine by purified recombinant VP1 of foot-and-mouth disease virus. Vaccine, 21: 3721-3729.
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      14. McCullough K C, Butcher R. 1982. Monoclonal antibodies against foot-and-mouth disease virus 146S and 12S particles. Arch Virol, 74 (1): 1-9.
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      16. Samir K R, Tamishraha B. 2008. Development and characterization of monoclonal antibodies against FMD virus type Asia-1 and determination of antigenic variations in the field strains. Vet Immunol Immunopathol, 122: 241-249.
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      Preparation and Characterization of Monoclonal Antibodies against VP1 Protein of Foot-and-mouth Disease Virus O/China99

        Corresponding author: Hui-yun CHANG, changhuiyun@126.com
      • 1. National FMD Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricutral Science, Lanzhou 730046, China
      • 2. Guangdong Open Laboratory of Veterinary Public Healty, Institute of Veterinary Medicine, Guangdong Academy of Agricultural Science, Guangzhou 510640, China
      • Received Date: 08 April 2009
      • Accepted Date: 15 September 2009

      Abstract: Monoclonal antibodies (McAbs) 1A9 and 9F12 against Foot-and-mouth disease virus (FMDV) serotype O were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with O/China99. Both McAbs reacted with O/China99 but not with Asia 1, as determined by immunohistochemistry assay. The microneutralization titer of the McAbs 1A9 and 9F12 were 640 and 1 280, respectively. Both McAbs contain kappa light chains, but the McAbs 1A9 and 9F12 were IgG1 and IgM, respectively. In order to define the McAbs binding epitopes, the reactivity of these McAbs against VP1, P20 and P14 were examined using indirect ELISA, the result showed that both McAbs reacted with VP1 and P20. McAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV.

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