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Volume 24 Issue 6
December 2009
    Citation: Wen-hua ZHAO, Shi-biao YANG, Jian-qiang HAN, Mei JIANG, Hua-chun LI, Nian-zu ZHANG, Qi-han LI. Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China .VIROLOGICA SINICA, 2009, 24(6) : 573-578.  http://dx.doi.org/10.1007/s12250-009-3064-x
    shu

    Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China

    cstr: 32224.14.s12250-009-3064-x
    • 1.

      Laboratory of Tropical and Subtropical Animal Virology of MOA, National Exotic Animal Disease Diagnostic Laboratory, Kunming 650224, China

    • 2.

      Yu Xi Agriculture Vocational Technical College, Yu Xi 653100, China

    • 3.

      Peking Union Medical College, Tsinghua University, Kunming Jiaoling Road 379#, Kunming 650118, China

    • Corresponding author: Shi-biao YANG, yangsb3799@sina.com
    • Received Date: 21 April 2009
      Accepted Date: 06 July 2009
      Available online: 01 December 2009

      Fund Project: Project from Yunnan Province 2008LA019project from MOA 2006-G57(3) B-Z1

    • This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%、97.3%、97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.
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      2. Bailey D, Banyard A, Dash P, et al. 2005. Full genome sequence of peste des petits ruminants virus, a member of the Morbillivirus genus. Virus Res, 110: 119-124.
          doi: 10.1016/j.virusres.2005.01.013

      3. Diallo A, Minet C, Le Goff C G, et al. 2007. The threat of peste des petits ruminants: progress in vaccine development for disease control. Vaccine, 25: 5591-5597.
          doi: 10.1016/j.vaccine.2007.02.013

      4. Food and farming group, International Trade Core Function. 2007. Peste des petits ruminants (PPR) in China. International Disease monitoring, Preminary outbreak assessment. Department for Environment Food and Rural Affairs, VITT 1200/BT-China, 27 July 2007. (http://www.defra.gov.uk/animalh/diseases/monitoring/pdf/ppr-china080714.pdf).

      5. Forsyth M A, Barrett T. 1995. Evaluation of polymerase chain reaction for the detection and characterization of rinderpest and peste des petits ruminants viruses for epidemiological studies. Virus Res, 39: 151-163.
          doi: 10.1016/0168-1702(95)00076-3

      6. Gargadennec L, Lalanne A. 1942. La peste des petits ruminants. Bulletin des Services Zoo Technoques et des Epizooties de I'Afrique Occidentale Francaise, 5: 16-21.

      7. Haffar A, Libeau G, Moussa A, et al.1999.The matrix protein gene sequence analysis reveals close relationship between peste des petits ruminants virus(PPRV)and dolphin morbillivirus. Virus Res, 64: 69-75.
          doi: 10.1016/S0168-1702(99)00080-5

      8. Jiang M, Yang S B, Zhang N Z. 2007. The epidemic trend and controlling of peste des petits ruminants(PPR). Prog Veter Med, 28 (supplement): 88-91. (in Chinese)

      9. Khan U Q M, Barrett T. 2008. Molecular based diagnosis of rinderpest and peste des petits ruminants virus in Pakistan. Int J Agri Biol, 10: 93-96.

      10. Kwiatek O, Minet C, Grillet C, et al. 2007. Peste des Petits Ruminants (PPR) Outbreak in Tajikistan. J Comp Path, 136: 111-119.
          doi: 10.1016/j.jcpa.2006.12.002

      11. Muthuchelvan D, Sanyal A, Sreenivasa B P, et al. 2006. Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus. Vet Microbiol, 113: 83-87.
          doi: 10.1016/j.vetmic.2005.10.014

      12. Pronab D, Sreenivasa B P, Barrett T, et al. 2002. Recent epidemiology of peste des petits ruminants virus (PPRV). Vet Microbiol, 88: 153-159.
          doi: 10.1016/S0378-1135(02)00102-5

      13. Senthil C K, Dhinakar G R, Thangavelu A, et al. 2007. Performance of RT-PCR-ELISA for the detection of peste des petits ruminants virus. Small Ruminant Research, 72: 200-208.
          doi: 10.1016/j.smallrumres.2006.09.004

      14. Yang S B, Zhao W H, Jiang M. 2008. Study of serological survey on the Peste des petits ruminants. Chin Anim Heal, 11: 63-65. (in Chinese)

      15. Zhao W H, Yang S B, Jiang M, et al. 2007. Sequencing and analysis on the N、M、F and H genes of PPRV. Prog Veter Med, 28 (11): 8-11. (in Chinese)

      16. Zhao W H, Zhu J B, Yao L T, et al. 2002. Cloning and sequencing of HN gene of a goose paramyxovirus isolate. Chin J Veter Sci Technol, 32: 10-13. (in Chinese)

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      Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China

        Corresponding author: Shi-biao YANG, yangsb3799@sina.com
      • 1. Laboratory of Tropical and Subtropical Animal Virology of MOA, National Exotic Animal Disease Diagnostic Laboratory, Kunming 650224, China
      • 2. Yu Xi Agriculture Vocational Technical College, Yu Xi 653100, China
      • 3. Peking Union Medical College, Tsinghua University, Kunming Jiaoling Road 379#, Kunming 650118, China
      • Received Date: 21 April 2009
      • Accepted Date: 06 July 2009
      Fund Project:  Project from Yunnan Province 2008LA019project from MOA 2006-G57(3) B-Z1

      Abstract: This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%、97.3%、97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.

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