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Volume 25 Issue 3
June 2010
    Citation: Xiao-wei MEI, Li YAO, Zhong-xin ZHANG. Localization and Functional Analysis of SeMNPV IE1 in Mammalian Cells .VIROLOGICA SINICA, 2010, 25(3) : 183-190.  http://dx.doi.org/10.1007/s12250-010-3113-5
    shu

    Localization and Functional Analysis of SeMNPV IE1 in Mammalian Cells

    • 1.

      State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • 2.

      Graduate school, Chinese Academy of Sciences, Beijing 100039, China

    • Corresponding author: Zhong-xin ZHANG, zhangzx@wh.iov.cn
    • Received Date: 19 November 2009
      Accepted Date: 02 March 2010
      Available online: 01 June 2010
    • In this paper, the function of the ie1 gene from baculovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), belonging to group II nucleopolyhedrovirus, was studied in mammalian cells. We amplified the SeMNPV ie1 gene and expressed it by fusing to the C terminal of enhanced GFP protein in HEK 293 cells. Confocal microscopy revealed that the IE1-GFP fusion protein was localized in the nucleus of the mammalian cells. The promoter sequences of AcMNPV gp64, SeMNPV F protein and Drosophila hsp70 were also analyzed, to further study the function of SeMNPV IE1. The results showed that, in the absence of the hr sequence, IE1 improved the expression of the F promoter but didn’t influence the gp64 promoter significantly, but IE1 moderately stimulated the hsp70 promoter.
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      Localization and Functional Analysis of SeMNPV IE1 in Mammalian Cells

        Corresponding author: Zhong-xin ZHANG, zhangzx@wh.iov.cn
      • 1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • 2. Graduate school, Chinese Academy of Sciences, Beijing 100039, China
      • Received Date: 19 November 2009
      • Accepted Date: 02 March 2010

      Abstract: In this paper, the function of the ie1 gene from baculovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), belonging to group II nucleopolyhedrovirus, was studied in mammalian cells. We amplified the SeMNPV ie1 gene and expressed it by fusing to the C terminal of enhanced GFP protein in HEK 293 cells. Confocal microscopy revealed that the IE1-GFP fusion protein was localized in the nucleus of the mammalian cells. The promoter sequences of AcMNPV gp64, SeMNPV F protein and Drosophila hsp70 were also analyzed, to further study the function of SeMNPV IE1. The results showed that, in the absence of the hr sequence, IE1 improved the expression of the F promoter but didn’t influence the gp64 promoter significantly, but IE1 moderately stimulated the hsp70 promoter.

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