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Volume 26 Issue 4
August 2011
    Citation: Tong Lin, Jing Li, Jun-jun Shao, Guo-zheng Cong, Jun-zheng Du, Shan-dian Gao, Hui-yun Chang. Develope Monoclonal Antibody against Foot-and-mouth Disease Virus A Type * .VIROLOGICA SINICA, 2011, 26(4) : 273-278.  http://dx.doi.org/10.1007/s12250-011-3199-4
    shu

    Develope Monoclonal Antibody against Foot-and-mouth Disease Virus A Type *

    cstr: 32224.14.s12250-011-3199-4

    • State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    • Corresponding author: Hui-yun Chang, changhuiyun@126.com
    • Received Date: 04 May 2011
      Accepted Date: 07 July 2011
      Available online: 01 August 2011

      Fund Project: State Key Projects of Transgene Program 2009ZX08006-002BState Key Projects of Transgene Program 2009ZX 08007-008BState Key Projects of Transgene Program 2011ZX08011-004

    • In order to develop an anti-FMDV A Type monoclonal antibody (mAb), BABL/c mice were immunized with FMDV A type. Monoclonal antibodies (mAbs) 7B11 and 8H4 against Foot-and-mouth disease virus (FMDV) serotype A were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with A/AV88. The microneutralization titer of the mAbs 7B11 and 8H4 were 1024 and 512, respectively. Both mAbs contain kappa light chains, the mAbs were IgG1. In order to define the mAbs binding epitopes, the reactivity of these mAbs against A Type FMDV, were examined using indirect ELISA, the result showed that both mAbs reacted with A Type FMDV. These mAbs may be used for further vaccine studies, diagnostic methods, prophylaxis, etiological and immunological research on FMDV. Characterization of these ncindicated that prepared ant i-FMDV A mAbs had no cross-reactivity with Swine Vesicular Disease (SVD) or FMDV O, Asia1 and C Type antigens. Their titers in abdomen liquor were 1:5×106 and 1:2×106, respectively. 7B11 was found to be of subtype IgG1, 8H4 was classified as IgG2b subtype. The mAbs prepared in this study, are specific for detection of FMDV serotype A, and is potentially useful for pen-side diagnosis.
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      1. Barteling S J. 2004. Modern inactivated foot-and mouth disease vaccines: historical background and key elements in production and use. In: Foot-and-Mouth Disease: Current Perspectives (Sobrino F, Domingo E. ed. ). England: Horizon Bioscience, p305–333.

      2. Du J Z, Chang H Y, Cong G Z, et al. 2005. Molecular characteristics and expression of structural protein gene of Foot-and-Mouth Disease virus type Asia1.Virol Sin, 20 (1):65-69.

      3. Freiberg B, Hohlich B, Haas B, et al. Type-independent detection of foot-and-mouth disease virus by monoclonal antibodies that bind to amino-terminal residues of capsid protein VP2. J Virol Methods, 92: 199-205.

      4. Feldmann M, Maini R N. 2001. Anti-TNF alpha therapy of rheumatoid arthritis: what have we learned? Annu Rev Immunol, 19:163-96.
          doi: 10.1146/annurev.immunol.19.1.163

      5. Guo H, Liu Z, Sun S, et al. 2005. Immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus O/China99.Vaccine, 23:3236-3242.
          doi: 10.1016/j.vaccine.2004.03.074

      6. Golding S M, Hedger R S. 1976. Radial immunodiffusion and serum neutralization techniques for the assay of antibodies to swine vesicular disease. Res Vet Sci, 20:142-147.

      7. Jackson T, King A M, Stuart D I, et al. 2003. Structure and receptor binding. Virus Res, 91:33-46.
          doi: 10.1016/S0168-1702(02)00258-7

      8. Klein J, Hussain M, Ahmad M, et al. 2007. Genetic characterisation of the recent foot-and-mouth disease virus subtype A/IRN/2005. Virol J, 4:122.
          doi: 10.1186/1743-422X-4-122

      9. Kovacs-Nolan J, Sasaki E, Yoo D, et al. 2001. Cloning and expression of human rotavirus spike protein, VP8*, in Escherichia coli. Biochem Biophys Res Commun, 282:1183-1188.
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      10. Lin T, Du J Z, Shao J J, et al. 2009. Application of VP1 Protein to Develop Monoclonal Virol Sin, 24(3): 215-220

      11. Lundberg M, Holmgren A, Johansson M. 2006. Human glutaredoxin 2 affinity tag for recombinant peptide and protein purification. Protein Expr Purif, 45:37-42.
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      12. Smith D B. 2000. Generating fusions to glutathione S-transferase for protein studies. Methods Enzymol, 326:254-270.
          doi: 10.1016/S0076-6879(00)26059-X

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      Develope Monoclonal Antibody against Foot-and-mouth Disease Virus A Type *

        Corresponding author: Hui-yun Chang, changhuiyun@126.com
      • State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
      • Received Date: 04 May 2011
      • Accepted Date: 07 July 2011
      Fund Project:  State Key Projects of Transgene Program 2009ZX08006-002BState Key Projects of Transgene Program 2009ZX 08007-008BState Key Projects of Transgene Program 2011ZX08011-004

      Abstract: In order to develop an anti-FMDV A Type monoclonal antibody (mAb), BABL/c mice were immunized with FMDV A type. Monoclonal antibodies (mAbs) 7B11 and 8H4 against Foot-and-mouth disease virus (FMDV) serotype A were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with A/AV88. The microneutralization titer of the mAbs 7B11 and 8H4 were 1024 and 512, respectively. Both mAbs contain kappa light chains, the mAbs were IgG1. In order to define the mAbs binding epitopes, the reactivity of these mAbs against A Type FMDV, were examined using indirect ELISA, the result showed that both mAbs reacted with A Type FMDV. These mAbs may be used for further vaccine studies, diagnostic methods, prophylaxis, etiological and immunological research on FMDV. Characterization of these ncindicated that prepared ant i-FMDV A mAbs had no cross-reactivity with Swine Vesicular Disease (SVD) or FMDV O, Asia1 and C Type antigens. Their titers in abdomen liquor were 1:5×106 and 1:2×106, respectively. 7B11 was found to be of subtype IgG1, 8H4 was classified as IgG2b subtype. The mAbs prepared in this study, are specific for detection of FMDV serotype A, and is potentially useful for pen-side diagnosis.

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