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Volume 27 Issue 1
February 2012
    Citation: Zhen-hua Yang, Ling Li, Zi-shu Pan. Development of Multiple ELISAs for the Detection of Antibodies against Classical Swine Fever Virus in Pig Sera .VIROLOGICA SINICA, 2012, 27(1) : 48-56.  http://dx.doi.org/10.1007/s12250-012-3227-z
    shu

    Development of Multiple ELISAs for the Detection of Antibodies against Classical Swine Fever Virus in Pig Sera

    cstr: 32224.14.s12250-012-3227-z

    • State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China

    • Corresponding author: Zi-shu Pan, zspan@whu.edu.cn
    • Received Date: 12 October 2011
      Accepted Date: 15 December 2011
      Available online: 01 February 2012

      Fund Project: the National Natural Science Foundation of China 30771597the Key Project of Science and Technology of Wuhan 200720422141

    • The major immunogenic proteins (Erns, E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E. coli and purified by affinity chromatography. The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies in pig sera. Optimum cut-off values were determined by receiver operating characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets. The multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization results. The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain vaccination.
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      Development of Multiple ELISAs for the Detection of Antibodies against Classical Swine Fever Virus in Pig Sera

        Corresponding author: Zi-shu Pan, zspan@whu.edu.cn
      • State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
      • Received Date: 12 October 2011
      • Accepted Date: 15 December 2011
      Fund Project:  the National Natural Science Foundation of China 30771597the Key Project of Science and Technology of Wuhan 200720422141

      Abstract: The major immunogenic proteins (Erns, E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E. coli and purified by affinity chromatography. The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies in pig sera. Optimum cut-off values were determined by receiver operating characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets. The multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization results. The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain vaccination.

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