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Citation: Chao Shan, Xiaodan Li, Chenglin Deng, Baodi Shang, Linlin Xu, Hanqing Ye, Zhiming Yuan, Bo Zhang. Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase [J].VIROLOGICA SINICA, 2013, 28(3) : 161-166.  http://dx.doi.org/10.1007/s12250-013-3332-7

Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase

  • Corresponding author: Zhiming Yuan, yzm@wh.iov.cn
    Bo Zhang, zhangbo@wh.iov.cn
  • Received Date: 09 April 2013
    Accepted Date: 13 May 2013
    Available online: 01 June 2013
  • We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.

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    Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase

      Corresponding author: Zhiming Yuan, yzm@wh.iov.cn
      Corresponding author: Bo Zhang, zhangbo@wh.iov.cn
    • Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese
    • Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of

    Abstract: We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.