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Volume 28 Issue 5
October 2013
    Citation: Tong Ou, Sanhua Li, Xiangyong Liao, Qiya Zhang. Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa .VIROLOGICA SINICA, 2013, 28(5) : 266-271.  http://dx.doi.org/10.1007/s12250-013-3340-7
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    Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa

    cstr: 32224.14.s12250-013-3340-7

    • State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

    • Corresponding author: Qiya Zhang, zhangqy@ihb.ac.cn
    • Received Date: 03 May 2013
      Accepted Date: 14 August 2013
      Published Date: 26 August 2013
      Available online: 01 October 2013
    • The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa, was isolated from Lake Dianchi,Kunming,China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24-48 hours and approximately 80 infectious units per cell,respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed four major structural proteins. These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.
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      Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa

        Corresponding author: Qiya Zhang, zhangqy@ihb.ac.cn
      • State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
      • Received Date: 03 May 2013
      • Accepted Date: 14 August 2013
      • Published Date: 26 August 2013

      Abstract: The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa, was isolated from Lake Dianchi,Kunming,China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24-48 hours and approximately 80 infectious units per cell,respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed four major structural proteins. These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.

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