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Volume 33 Issue 2
April 2018
    Citation: Zhichao Xu, Huiling Zhong, Qingfeng Zhou, Yunping Du, Li Chen, Yun Zhang, Chunyi Xue, Yongchang Cao. A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets .VIROLOGICA SINICA, 2018, 33(2) : 131-141.  http://dx.doi.org/10.1007/s12250-018-0003-8
    shu

    A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets

    • 1.

      State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510006, China

    • 2.

      Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd, Xinxing 527400, China

    • 3.

      School of Life Science, Higher Education Mega Center, Sun Yat-sen University, Guangzhou 510006, China

    • Corresponding author: Yongchang Cao, caoych@mail.sysu.edu.cn
    • Received Date: 20 June 2017
      Accepted Date: 22 November 2017
      Published Date: 22 March 2018
      Available online: 01 April 2018
    • Porcine deltacoronavirus (PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and mainland China. Here we isolated a strain of PDCoV, named CHN-GD-2016, from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1×105 TCID50 of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong, China and is highly pathogenic in newborn piglets.
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      1. Chen F, Zhu Y, Wu M, Ku X, Yao L, He Q (2015a) Full-length genome characterization of Chinese porcine deltacoronavirus strain CH/SXD1/2015. Genome Announc 3:e01284

      2. Chen Q, Gauger P, Stafne M, Thomas J, Arruda P, Burrough E, Madson D, Brodie J, Magstadt D, Derscheid R, Welch M, Zhang J (2015b) Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets. Virology 482:51–59
          doi: 10.1016/j.virol.2015.03.024

      3. Chen Q, Gauger PC, Stafne MR, Thomas JT, Madson DM, Huang H, Zheng Y, Li G, Zhang J (2016) Pathogenesis comparison between the United States porcine epidemic diarrhoea virus prototype and S-INDEL-variant strains in conventional neonatal piglets. J Gen Virol 97:1107–1121
          doi: 10.1099/jgv.0.000419

      4. Dong N, Fang L, Yang H, Liu H, Du T, Fang P, Wang D, Chen H, Xiao S (2016) Isolation, genomic characterization, and pathogenicity of a Chinese porcine deltacoronavirus strain CHN-HN-2014. Vet Microbiol 196:98–106
          doi: 10.1016/j.vetmic.2016.10.022

      5. Doyle LP, Hutchings LM (1946) A transmissible gastroenteritis in pigs. J Am Vet Med Assoc 108:257–259

      6. Hu H, Jung K, Vlasova AN, Chepngeno J, Lu Z, Wang Q, Saif LJ (2015a) Isolation and characterization of porcine deltacoronavirus from pigs with diarrhea in the United States. J Clin Microbiol 53:1537–1548
          doi: 10.1128/JCM.00031-15

      7. Hu X Jr, Li N Jr, Tian Z Jr, Yin X Jr, Qu L, Qu J (2015b) Molecular characterization and phylogenetic analysis of transmissible gastroenteritis virus HX strain isolated from China. BMC Vet Res 11:72
          doi: 10.1186/s12917-015-0387-8

      8. Hu H, Jung K, Vlasova AN, Saif LJ (2016) Experimental infection of gnotobiotic pigs with the cell-culture-adapted porcine deltacoronavirus strain OH-FD22. Arch Virol 161:3421–3434
          doi: 10.1007/s00705-016-3056-8

      9. Hull RN, Cherry WR, Weaver GW (1976) The origin and characteristics of a pig kidney cell strain, LLC-PK. In Vitro 12:670–677
          doi: 10.1007/BF02797469

      10. Jeong YJ, Park SI, Hosmillo M, Shin DJ, Chun YH, Kim HJ, Kwon HJ, Kang SY, Woo SK, Park SJ, Kim GY, Kang MI, Cho KO (2009) Detection and molecular characterization of porcine group C rotaviruses in South Korea. Vet Microbiol 138:217–224
          doi: 10.1016/j.vetmic.2009.03.024

      11. Jung K, Hu H, Eyerly B, Lu Z, Chepngeno J, Saif LJ (2015) Pathogenicity of 2 porcine deltacoronavirus strains in gnotobiotic Pigs. Emerg Infect Dis 21:650–654
          doi: 10.3201/eid2104.141859

      12. Kong Q, Xue C, Ren X, Zhang C, Li L, Shu D, Bi Y, Cao Y (2013) Proteomic analysis of purified coronavirus infectious bronchitis virus particles. Proteomic Sci 8:29

      13. Lee S, Lee C (2014) Complete genome characterization of korean porcine deltacoronavirus strain KOR/KNU14-04/2014. Genome Announc 2:e01191

      14. Ma Y, Zhang Y, Liang X, Lou F, Oglesbee M, Krakowka S, Li J (2015) Origin, evolution, and virulence of porcine deltacoronaviruses in the United States. Mbio 6:e00064

      15. Madson DM, Magstadt DR, Arruda PH, Hoang H, Sun D, Bower LP, Bhandari M, Burrough ER, Gauger PC, Pillatzki AE, Stevenson GW, Wilberts BL, Brodie J, Harmon KM, Wang C, Main RG, Zhang J, Yoon KJ (2014) Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs. Vet Microbiol 174:60–68
          doi: 10.1016/j.vetmic.2014.09.002

      16. Malboeuf CM, Yang X, Charlebois P, Qu J, Berlin AM, Casali M, Pesko KN, Boutwell CL, Devincenzo JP, Ebel GD, Allen TM, Zody MC, Henn MR, Levin JZ (2013) Complete viral RNA genome sequencing of ultra-low copy samples by sequenceindependent amplification. Nucleic Acids Res 41:e13
          doi: 10.1093/nar/gks794

      17. Marthaler D, Raymond L, Jiang Y, Collins J, Rossow K, Rovira A (2014) Rapid detection, complete genome sequencing, and phylogenetic analysis of porcine deltacoronavirus. Emerg Infec Dis 20:1347–1350

      18. Pensaert MB, De Bouck PD (1978) A new coronavirus-like particle associated with diarrhea in swine. Arch Virol 58:243–247
          doi: 10.1007/BF01317606

      19. Reed LJ, Muench H (1938) A simple method of estimating of fifty per cent endpoints. Am J Epidemiol 27:493–497
          doi: 10.1093/oxfordjournals.aje.a118408

      20. Saeng-Chuto K, Lorsirigool A, Temeeyasen G, Vui DT, Stott CJ, Madapong A, Tripipat T, Wegner M, Intrakamhaeng M, Chongcharoen W, Tantituvanont A, Kaewprommal P, Piriyapongsa J, Nilubol D (2017) Different lineage of porcine deltacoronavirus in Thailand, Vietnam and Lao PDR in 2015. Transbound Emerg Dis 64:3–10
          doi: 10.1111/tbed.2017.64.issue-1

      21. Sasseville AM, Boutin M, Gé linas AM, Dea S (2002) Sequence of the 30-terminal end (8.1 kb) of the genome of porcine haemagglutinating encephalomyelitis virus: comparison with other haemagglutinating coronaviruses. J Gen Virol 83:2411–2416

      22. Song D, Zhou X, Peng Q, Chen Y, Zhang F, Huang T, Zhang T, Li A, Huang D, Wu Q, He H, Tang Y (2015) Newly emerged porcine deltacoronavirus associated with diarrhoea in swine in China: identification, prevalence and full-length genome sequence analysis. Transbound Emerg Dis 62:575–580
          doi: 10.1111/tbed.2015.62.issue-6

      23. Su M, Li C, Guo D, Wei S, Wang X, Geng Y, Yao S, Gao J, Wang E, Zhao X, Wang Z, Wang J, Wu R, Feng L, Sun D (2016) A recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbentassay to detect antibodies against porcine deltacoronavirus. J Vet Med Sci 78:601–606
          doi: 10.1292/jvms.15-0533

      24. Wang L, Byrum B, Zhang Y (2014a) Detection and genetic characterization of deltacoronavirus in pigs, Ohio, USA, 2014. Emerg Infect Dis 20:1227–1230

      25. Wang L, Byrum B, Zhang Y (2014b) Porcine coronavirus HKU15 detected in 9 US States, 2014. Emerg Infect Dis 20:1594–1595
          doi: 10.3201/eid2009.140756

      26. Wang YW, Yue H, Fang W, Huang YW (2015) Complete genome sequence of porcine deltacoronavirus strain CH/Sichuan/S27/ 2012 from Mainland China. Genome Announc 3:e00945

      27. Wesley RD, Woods RD, Hill HT, Biwer JD (1990) Evidence for a porcine respiratory coronavirus, antigenically similar to transmissible gastroenteritis virus, in the United States. J Vet Diagn Invest 2:312–317
          doi: 10.1177/104063879000200411

      28. Woo PCY, Yi H, Lau SKP, Kwok-Yung Y (2010) Coronavirus genomics and bioinformatics analysis. Viruses 2:1804–1820
          doi: 10.3390/v2081803

      29. Woo PC, Lau SK, Lam CS, Lau CC, Tsang AK, Lau JH, Bai R, Teng JL, Tsang CC, Wang M, Zheng BJ, Chan KH, Yuen KY (2012) Discovery of seven novel mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus. J Virol 86:3995–4008
          doi: 10.1128/JVI.06540-11

      30. Zhai SL, Wei WK, Li XP, Wen XH, Zhou X, Zhang H, Lv DH, Li F, Wang D (2016) Occurrence and sequence analysis of porcine deltacoronaviruses in southern China. Virol J 13:136
          doi: 10.1186/s12985-016-0591-6

      31. Zhang J (2016) Porcine deltacoronavirus: overview of infection dynamics, diagnostic methods, prevalence and genetic evolution. Virus Res 226:71–84
          doi: 10.1016/j.virusres.2016.05.028

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      A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets

        Corresponding author: Yongchang Cao, caoych@mail.sysu.edu.cn
      • 1. State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510006, China
      • 2. Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd, Xinxing 527400, China
      • 3. School of Life Science, Higher Education Mega Center, Sun Yat-sen University, Guangzhou 510006, China
      • Received Date: 20 June 2017
      • Accepted Date: 22 November 2017
      • Published Date: 22 March 2018

      Abstract: Porcine deltacoronavirus (PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and mainland China. Here we isolated a strain of PDCoV, named CHN-GD-2016, from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1×105 TCID50 of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong, China and is highly pathogenic in newborn piglets.

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