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Citation: Yang Han, Qingyu Yang, Ying Liu, Ting Shu, Li Yue, Ting Xiao, Qin Zeng, Ying Wu, Xi Zhou, Dingyu Zhang. Feasibility Study of Mixing Throat Swab Samples for Severe Acute Respiratory Syndrome Coronavirus-2 Screening [J].VIROLOGICA SINICA, 2020, 35(6) : 830-832.  http://dx.doi.org/10.1007/s12250-020-00254-x

Feasibility Study of Mixing Throat Swab Samples for Severe Acute Respiratory Syndrome Coronavirus-2 Screening

  • Corresponding author: Xi Zhou, zhangdy63@hotmail.com, ORCID: http://orcid.org/0000-0002-3846-5079
    Dingyu Zhang, zhouxi@wh.iov.cn, ORCID: http://orcid.org/0000-0002-9277-5705
  • Received Date: 12 May 2020
    Accepted Date: 03 June 2020
    Published Date: 08 July 2020
    Available online: 01 December 2020
  • At present, viral nucleic acids are generally sampled using throat swabs and detected by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). However, the capacity for SARS-CoV-2 nucleic acid detection is largely limited by the number of instruments, kits, and experienced laboratory personnel available. These limitations have caused low screening efficiency, which leads to a lag in identifying potential infections and may then exacerbate the spread of COVID-19 (Carter et al. 2020). Therefore, novel qRT-PCR approaches for nucleic acid detection are required to enhance testing efficiency.In this study, we aimed to explore a practical method and procedure for SARS-CoV-2 qRT-PCR detection in 96-well plates using pooled throat swab samples. This method may reduce the cost of testing and increase the screening capacity for SARS-CoV-2 using existing instrument and kits, consequently facilitating the containment of COVID-19 worldwide.

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    Feasibility Study of Mixing Throat Swab Samples for Severe Acute Respiratory Syndrome Coronavirus-2 Screening

      Corresponding author: Xi Zhou, zhangdy63@hotmail.com
      Corresponding author: Dingyu Zhang, zhouxi@wh.iov.cn
    • 1. Center for Translational Medicine, Wuhan Jinyintan Hospital, Wuhan 430023, China
    • 2. State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan 430071, China
    • 3. Joint Laboratory of Infectious Diseases and Health, Wuhan Institute of Virology and Wuhan Jinyintan Hospital, CAS, Wuhan 430023, China
    • 4. State Key Laboratory of Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430072, China

    Abstract: At present, viral nucleic acids are generally sampled using throat swabs and detected by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). However, the capacity for SARS-CoV-2 nucleic acid detection is largely limited by the number of instruments, kits, and experienced laboratory personnel available. These limitations have caused low screening efficiency, which leads to a lag in identifying potential infections and may then exacerbate the spread of COVID-19 (Carter et al. 2020). Therefore, novel qRT-PCR approaches for nucleic acid detection are required to enhance testing efficiency.In this study, we aimed to explore a practical method and procedure for SARS-CoV-2 qRT-PCR detection in 96-well plates using pooled throat swab samples. This method may reduce the cost of testing and increase the screening capacity for SARS-CoV-2 using existing instrument and kits, consequently facilitating the containment of COVID-19 worldwide.