Construction of a New Kind of Recombinant Adenovirus and Its effect on Tnmor Cells

E1A gene of adenovirus is an apoptosis-inducing gene and E1B gene of adenovirus is anapoptosis-inhibiting gene. The former one was cloned into adenovirus transfer vector pCA13 under the control of HCMV IE1 promoter, which resulted into transfer vector pCAE1A. PlasmidpCAE1A and pBHG11, which includes almost all the adenovirus genome but E1 region and E3 region were deleted, were cotransfected into 293 cell line. After 7-10 days, recombinant virusv5Ad4(E1B- E3- ) was obtained. v5Ad4 was used to infect A549 cell line. The results showedthat v5Ad4 can kill and lysis tumor cells. In the normal cells, v5Ad4 didn' t show visible cytotoxicity effect.