Citation: LEI Wan-Li, TANG Jia-Qi, LU Cheng-Beng, PAN Xiu-Zhen, LI Xian-Fu. Detection and Genotyping of Hantaviruses Using Reverse Transcription·Polym erase Chain Reaction .VIROLOGICA SINICA, 1998, 13(1) : 28.

Detection and Genotyping of Hantaviruses Using Reverse Transcription·Polym erase Chain Reaction

  • Available online: 05 March 1998
  • Common primers were designed, based on M segmental sequences of two HFRSV serotypes—HTNV and SEOV, and RT PCR (reverse transcription-polymerase chain reaction) method was applied in detecting 39 strains of isolated hosts from different areas. Thirty six of the 39 samples were tested by cELISA (capture ELISA) in duplicated wells, while P/N≥2.10 and P-N≥0.10 the result was considered to the positive. The detection rates of RT PCR and cELISA were 97.6% and 82.4% respectively, 84.6% in correspondence. Fifteen of 38 RT PCR products could be digested by restriction endonuclease AluI. According to the restriction map the HFRSV detected was classified into two types——HTNV and SEOV.

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    Detection and Genotyping of Hantaviruses Using Reverse Transcription·Polym erase Chain Reaction

    • 1. East-China Medical Istitute of Biological Techniques,Nanjing 210002College of Veterinary Medicine,Nanjing Agrieultural University.Najing 210095

    Abstract: Common primers were designed, based on M segmental sequences of two HFRSV serotypes—HTNV and SEOV, and RT PCR (reverse transcription-polymerase chain reaction) method was applied in detecting 39 strains of isolated hosts from different areas. Thirty six of the 39 samples were tested by cELISA (capture ELISA) in duplicated wells, while P/N≥2.10 and P-N≥0.10 the result was considered to the positive. The detection rates of RT PCR and cELISA were 97.6% and 82.4% respectively, 84.6% in correspondence. Fifteen of 38 RT PCR products could be digested by restriction endonuclease AluI. According to the restriction map the HFRSV detected was classified into two types——HTNV and SEOV.

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