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Citation: Li-na ZHU, Chun-lei MEI, Zhong-xin ZHANG. The Function of SeMNPV IAP3 in Mammalian Cells [J].VIROLOGICA SINICA, 2008, 23(3) : 183-188.  http://dx.doi.org/10.1007/s12250-008-2883-5

The Function of SeMNPV IAP3 in Mammalian Cells

  • Corresponding author: Zhong-xin ZHANG, zhangzx@wh.iov.cn
  • Received Date: 25 May 2007
    Accepted Date: 04 January 2008
    Available online: 01 June 2008
  • The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I /Bam H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.

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    The Function of SeMNPV IAP3 in Mammalian Cells

      Corresponding author: Zhong-xin ZHANG, zhangzx@wh.iov.cn
    • 1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
    • 2. Graduate school, Chinese Academy of Sciences, Beijing 100039, China

    Abstract: The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I /Bam H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.