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Citation: Xue-en FANG, Jian LI, Qin CHEN. One New Method of Nucleic Acid Amplification —Loop-mediated Isothermal Amplification of DNA [J].VIROLOGICA SINICA, 2008, 23(3) : 167-172.  http://dx.doi.org/10.1007/s12250-008-2929-8

One New Method of Nucleic Acid Amplification —Loop-mediated Isothermal Amplification of DNA

  • Corresponding author: Qin CHEN, chenqincc@yahoo.com.cn
  • Received Date: 17 December 2007
    Accepted Date: 31 January 2008
    Available online: 01 June 2008

    Fund Project: Grant support from General Administration of Quality Supervision, Inspection and Quarantine of China HK001-2007

  • Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.

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    One New Method of Nucleic Acid Amplification —Loop-mediated Isothermal Amplification of DNA

      Corresponding author: Qin CHEN, chenqincc@yahoo.com.cn
    • 1. School of Life Science, Shanghai University, Shanghai 200444, China
    • 2. Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China
    Fund Project:  Grant support from General Administration of Quality Supervision, Inspection and Quarantine of China HK001-2007

    Abstract: Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.