In recent years, a large number of new and previously identfied viruses are emerging or re-emerging, such as SARS coronavirus, Avian influenza A virus and Nipah virus (7, 11, 14, 19), which seriously threaten the health of the general population. Rapid identification of these viruses plays a vital role in the diagnosis and control of their corresponding diseases; therefore increasing effort is being placed on the technology for their isolation and identification (3, 4, 5, 9, 16, 19). Immunological techniques, especially the development of monoclonal antibodies have provided powerful tools to detect viral particles (3, 4, 16). Molecular detection of viral DNA and RNA is routinely used to rapidly detect and identify known pathogens (1, 2, 4, 19). Compared to traditional cell culture methods, these molecular methods have the advantages of being rapid and simple to use. However, cell culture is still regarded as "the golden standard" for viral isolation, and is also one of the most convincing methods in virus identifi-cation (4). The discovery by John Enders, Thomas Weller, and Frederick Robbins that poliovirus could propagate in cultured cells in 1949 was regarded as a revolutionary finding, for which these three scientists were awarded the Nobel Prize in physiology and medicine in 1954. Their work stimulated the amplifi-cation of many other viruses in cultured cells and also facilitated the discovery of new viruses and the development of many viral vaccines (4, 9, 11, 14). Traditionally, viral isolation by cell culture normally uses individual cell lines for viral infection. This approach becomes less effective when there is a limited supply of specimen and a number of different cell lines have to be used for isolation of novel or unknown viruses. To this end, we have developed a new isolated co-culture cell system which allows virus isolation in multiple cell lines using a small amount of starting materials.
An Improved Culture System for Virus Isolation and Detection*
- Received Date: 14 April 2008
- Accepted Date: 25 July 2008
Abstract: Abstract: Cell culture has played an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology study of viruses. In the present study, a new system that could support multiple different cell lines to be simultaneous cultured in one dish was developed. In the system, each cell line was cultured in an isolated zone in the same dish or well and the system is therefore called isolated co-cultured system. The usefulness of this novel approach for virus isolation was demonstrated using a model system based on adenovirus.