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Volume 24 Issue 1
February 2009
    Citation: Shao-hui MA, Jian-sheng LIU, Hai-jing SHI, Li-chun WANG, Jing-jing WANG, Long-ding LIU, Qi-han LI. Complete Nucleotide Sequence of a Mumps Virus SP Strain Isolated in China* .VIROLOGICA SINICA, 2009, 24(1) : 28-36.  http://dx.doi.org/10.1007/s12250-009-2984-9
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    Complete Nucleotide Sequence of a Mumps Virus SP Strain Isolated in China*

    cstr: 32224.14.s12250-009-2984-9

    • Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China

    • Corresponding author: Qi-han LI, qihanli@21cn.com
    • Received Date: 04 August 2008
      Accepted Date: 18 December 2008
      Available online: 01 February 2009

      Fund Project: Natural Science Foundation of Yunnan province 2008CD153Public Benefit Grant of Ministry of Health, China 200802035

    • The complete nucleotide sequence of the mumps virus SP, which was isolated in China, was determined. As with other mumps viruses, its genome was 15 384 nucleotides (nts) in length and encoded seven proteins. The full-length nucleotide sequence of the SP isolate differed from other strains by 4% –6.8% at the nucleotide sequence level. Due to variations of amino acids over the full genome (including the HN and N genes), this isolate exhibited significant variations in the antigenic sites. This report is the first to describe the full-length genome of a genotype F strain and provide an overview of the diversity of genetic characteristics of a circulating mumps virus.
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      1. Brown E G, Dimock K, Wright K E. 1996. The Urabe AM9 mumps vaccine is a mixture of viruses differing at amino acid 335 of the hemagglutinin-neuraminidase gene with one form associated with disease. J Infect Dis, 174 (3): 619-622.
          doi: 10.1093/infdis/174.3.619

      2. Elliott G D, Afzal M A, Martin S, et al. 1989. Nucleotide sequence of the matrix, fusion and putative SH protein genes of mumps virus and their deduced amino acid sequences. Virus Res, 12 (1): 61-67.
          doi: 10.1016/0168-1702(89)90054-3

      3. Elliott G D, Yeo R P, Afzal M A, et al. 1990. Strain-variable editing during transcription of the P gene of mumps virus may lead to the generation of non-structural proteins NS1 (V) and NS2. J Gen Virol, 71 (Pt 7): 1555- 1560.

      4. Ivancic J, Gulija T K, Forcic D, et al. 2005. Genetic characterization of L- Zagreb mumps vaccine strain. Virus Res, 109: 95-105.
          doi: 10.1016/j.virusres.2004.11.013

      5. Ivancic J J, Santak M, Forcic D. 2008. Variability of hemagglutinin-neuraminidase and nucleocapsid protein of vaccine and wild-type mumps virus strains. Infect Genet Evol, 8: 603-613.
          doi: 10.1016/j.meegid.2008.04.007

      6. Jin L, Beard S, Hale A, et al.2000. The genomic sequence of a contemporary wild-type mumps virus strain. Virus Res, 70 (1-2): 75-83.
          doi: 10.1016/S0168-1702(00)00211-2

      7. Lee J Y, Na B K, Lee H D, et al. 2004. Complete nucleotide sequence of a mumps virus genotype I strain isolated in Korea. Virus Genes, 28: 201-205.
          doi: 10.1023/B:VIRU.0000016859.04203.88

      8. Ma S H, Zhang R S, Liu L D, et al. 2006. Sequence analysis of the SH gene and its flanking region of mumps virus isolated strain (SP strain). J Micro Infect, 1: 87-90. (in Chinese)

      9. Muhlemann K. 2004. The molecular epidemiology of mumps virus. Infect Genet Evol, 4: 215-219.
          doi: 10.1016/j.meegid.2004.02.003

      10. Orvell C, Kalantari M, Johansson B. 1997. Characteri- zation of five conserved genotypes of the mumps virus small hydrophobic (SH) protein gene. J Genl Virol, 78: 91-95.
          doi: 10.1099/0022-1317-78-1-91

      11. Rubin S A, Amexis G, Pletnikov M, et al. 2003. Changes in mumps virus gene sequence associated with variability in neurovirulent phenotype. J Virol, 77: 11616-11624.
          doi: 10.1128/JVI.77.21.11616-11624.2003

      12. Santos C L S, Ishida M A, Foster P G, et al. 2008. Detection of a new mumps virus genotype during parotitis epidemic of 2006-2007 in the state of Sao Paulo, Brazil. J Med Virol, 80: 323-329.
          doi: 10.1002/(ISSN)1096-9071

      13. Santak M, Kosutic-Gulija T, Tseovic G, et al. 2006. Mumps virus strains isolated in Croatia in 1998 and 2005: Genotyping and putative antigenic relatedness to vaccine strains. J Med Virol, 78: 638-643.
          doi: 10.1002/(ISSN)1096-9071

      14. Tecle T, Johansson B, Jejcic A, et al. 1998. Characteri- zation of three co-circulating genotypes of the small hydro- phobic protein gene of mumps virus, J Gen Virol, 79: 2929-2937.
          doi: 10.1099/0022-1317-79-12-2929

      15. Tecle T, Mickien A, Johansson B, et al. 2002. Molecular characterization of two mumps virus genotypes circulating during an epidemic in Lithuania from 1998 to 2000, Arch Virol, 147: 243-253.
          doi: 10.1007/s705-002-8317-y

      16. Wolinsky J S, Waxham M N, Server A C. 1985. Protective effects of glycoprotein-specifi monoclonal anti- bodies on the course of experimental mumps virus menin- goencephalitis. J Virol, 53: 727-734.

      17. Wu L, Bai Z, Li Y, et al. 1998. Wild type mumps viruses circulating in China establish a new genotype. Vaccine, 16: 281-285.
          doi: 10.1016/S0264-410X(97)00173-4

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      Complete Nucleotide Sequence of a Mumps Virus SP Strain Isolated in China*

        Corresponding author: Qi-han LI, qihanli@21cn.com
      • Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China
      • Received Date: 04 August 2008
      • Accepted Date: 18 December 2008
      Fund Project:  Natural Science Foundation of Yunnan province 2008CD153Public Benefit Grant of Ministry of Health, China 200802035

      Abstract: The complete nucleotide sequence of the mumps virus SP, which was isolated in China, was determined. As with other mumps viruses, its genome was 15 384 nucleotides (nts) in length and encoded seven proteins. The full-length nucleotide sequence of the SP isolate differed from other strains by 4% –6.8% at the nucleotide sequence level. Due to variations of amino acids over the full genome (including the HN and N genes), this isolate exhibited significant variations in the antigenic sites. This report is the first to describe the full-length genome of a genotype F strain and provide an overview of the diversity of genetic characteristics of a circulating mumps virus.

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