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Volume 24 Issue 1
February 2009
    Citation: Zhi-xun XIE, Jian-bao DONG, Xiao-fei TANG, Jia-bo LIU, Yao-shan PANG, Xian-wen DENG, Zhi-qin XIE, Li-ji XIE, Mazhar I Khan. Genome Sequencing and Phylogenetic Analysis of Three Avian Influenza H9N2 Subtypes in Guangxi* .VIROLOGICA SINICA, 2009, 24(1) : 37-44.  http://dx.doi.org/10.1007/s12250-009-2985-8
    shu

    Genome Sequencing and Phylogenetic Analysis of Three Avian Influenza H9N2 Subtypes in Guangxi*

    • 1.

      Guangxi Veterinary Research Institute, Nanning 530001, China

    • 2.

      University of Connecticut, Storrs CT06269-3089, USA

    • Corresponding author: Zhi-xun XIE, xiezhixun@126.com
    • Received Date: 04 August 2008
      Accepted Date: 08 December 2008
      Available online: 01 February 2009

      Fund Project: Guangxi Science Techno- logy Bureau 0719004-3ANational Bai Qian Wan Talents Eng-ineering Foudation 945200603

    • Three isolates of H9N2 Avian Influenza viruses (AIV) were isolated from chickens in Guangxi province. Eight pairs of specific primers were designed and synthesized according to the sequences of H9N2 at GenBank. phylogenetic analysis showed a high degree of homology between the Guangxi isolates and isolates from Guangdong and Jiangsu provinces, suggesting that the Guangxi isolates originated from the same source. However, the eight genes of the three isolates from Guangxi were not in the same sublineages in their respective phylogenetic trees, which suggests that they were products of natural reassortment between H9N2 avian influenza viruses from different sublineages. The 9 nucleotides ACAGAGATA which encode amino acids T, G, I were absent between nucleotide 205 and 214 in the open reading frame of the NA gene in the Guangxi isolates. AIV strains that infect human have, in their HA proteins, leucine at position 226. The analysis of deduced amino acid sequence of HA proteins showed that position 226 of these isolates contained glycine instead of leucine, suggesting that these three isolates differ from H9N2 AIV strains isolated from human infections.
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      4. Guan Y, Shortridge K F, Krauss S, et al. 2000.H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China. J Virol, 74 (20): 9372-9380.
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      6. Kwon H J, Cho S H, Kim M C, et al. 2006. Molecular epizootiology of recurrent low pathogenic avian influenza by H9N2 subtype virus in Korea. Avian Pathol, 35 (4): 309-315.
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      7. Li K S, Xu K M, Peiris S M, et al. 2003. Characterization of H9 subtype influenza viruses from the ducks of southern China: a candidate for the next influenza pandemic in humans?. J Virol, 77 (12): 6988-6994.
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      8. Lin Y P, Shaw M, Gregory V, et al. 2000. Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates. Proc Natl Acad Sci U S A, 97 (17): 9654-9658.
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      9. Liu H, Liu X F, Cheng J, Peng D, et al. 2003. Phy-logenetic analysis of the hemagglutinin genes of twentysix avian influenza viruses of subtype H9N2 isolated from chickens in China during 1996-2001. Avian Dis, 47 (1): 116-127.
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      10. Liu J H, Okazaki K, Shi W M, et al. 2003.Phylogenetic analysis of neuraminidase gene of H9N2 influenza viruses prevalent in chickens in China during 1995-2002. Virus Genes, 27 (2): 197-202.
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      Genome Sequencing and Phylogenetic Analysis of Three Avian Influenza H9N2 Subtypes in Guangxi*

        Corresponding author: Zhi-xun XIE, xiezhixun@126.com
      • 1. Guangxi Veterinary Research Institute, Nanning 530001, China
      • 2. University of Connecticut, Storrs CT06269-3089, USA
      • Received Date: 04 August 2008
      • Accepted Date: 08 December 2008
      Fund Project:  Guangxi Science Techno- logy Bureau 0719004-3ANational Bai Qian Wan Talents Eng-ineering Foudation 945200603

      Abstract: Three isolates of H9N2 Avian Influenza viruses (AIV) were isolated from chickens in Guangxi province. Eight pairs of specific primers were designed and synthesized according to the sequences of H9N2 at GenBank. phylogenetic analysis showed a high degree of homology between the Guangxi isolates and isolates from Guangdong and Jiangsu provinces, suggesting that the Guangxi isolates originated from the same source. However, the eight genes of the three isolates from Guangxi were not in the same sublineages in their respective phylogenetic trees, which suggests that they were products of natural reassortment between H9N2 avian influenza viruses from different sublineages. The 9 nucleotides ACAGAGATA which encode amino acids T, G, I were absent between nucleotide 205 and 214 in the open reading frame of the NA gene in the Guangxi isolates. AIV strains that infect human have, in their HA proteins, leucine at position 226. The analysis of deduced amino acid sequence of HA proteins showed that position 226 of these isolates contained glycine instead of leucine, suggesting that these three isolates differ from H9N2 AIV strains isolated from human infections.

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