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Volume 24 Issue 3
June 2009
    Citation: Hua-qi PAN, Nan WANG, Li LIU, Lei LIU, Jiang-chun HU, Pu-yan CHEN, Shu-jin WANG, Rui-bing CAO. Molecular Characterization of the Duck Enteritis Virus UL4 Gene .VIROLOGICA SINICA, 2009, 24(3) : 171-178.  http://dx.doi.org/10.1007/s12250-009-3002-y
    shu

    Molecular Characterization of the Duck Enteritis Virus UL4 Gene

    cstr: 32224.14.s12250-009-3002-y
    • 1.

      Key Laboratory of Animal Disease Diagnosis and Immunology, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China

    • 2.

      Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016, China

    • 3.

      College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

    • Corresponding author: Rui-bing CAO, crb@njau.edu.cn
    • Received Date: 16 September 2008
      Accepted Date: 16 January 2009
      Available online: 01 June 2009
    • Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious and fatal disease. In the present article, the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction (PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame (ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups, and the duck enteritis virus branched separately, closely related to the Mardiviruses group comprising Gallid herpesvirus 2 (GaHV-2), Gallid herpesvirus 3 (GaHV-3) and Meleagrid herpesvirus 1 (MeHV-1). The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.
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      1. Afonso C L, Tulman E R, Lu Z, et al. 2001. The genome of turkey herpesvirus. J Virol, 75: 971-978.
          doi: 10.1128/JVI.75.2.971-978.2001

      2. Baines J D, Roizman B. 1991. The open reading frames UL3, UL4, UL10, and UL16 are dispensable for the replication of herpes simplex virus 1 in cell culture. J Virol, 65: 938-944.

      3. Brewster F K, Vladimir Z, Juraj K, et al. 2001. The genome of herpesvirus of turkeys: comparative analysis with Marek's disease viruses. J Gen Virol, 82: 1123-1135.
          doi: 10.1099/0022-1317-82-5-1123

      4. Davidson A J, Scott J E. 1986. The complete DNA sequence of varicella-zoster virus. J Gen Virol, 67: 1759-1816.
          doi: 10.1099/0022-1317-67-9-1759

      5. Dean H J, Cheung A K. 1994. Identification of the pseudorabies virus UL4 and UL5 (helicase) genes. Virology, 202: 962-967.
          doi: 10.1006/viro.1994.1419

      6. Eide T, Marsden H S, Leib D A, et al. 1998. Identifi-cation of the UL4 protein of herpes simplex virus type 1. J Gen Virol, 79: 3033-3038.
          doi: 10.1099/0022-1317-79-12-3033

      7. Fuchs W, Granzow H, Klopfleisch R, et al. 2006. The UL4 gene of pseudorabies virus encodes a minor infected-cell protein that is dispensable for virus replication. J Gen Virol, 87(9): 2517-2525.
          doi: 10.1099/vir.0.81813-0

      8. Fuchs W, Mettenleiter T C. 1996. DNA sequence and transcriptional analysis of the UL1 to UL5 gene cluster of infectious laryngotracheitis virus. J Gen Virol, 77: 2221-2229.
          doi: 10.1099/0022-1317-77-9-2221

      9. Hansen W R, Nashold S W, Docherty D E, et al. 2000. Diagnosis of duck plague in waterfowl by polymerase chain reaction. Avian Dis, 44 (2): 266-274.
          doi: 10.2307/1592539

      10. Jun P Y, Strelow L I, Herman R C, et al. 1998. The UL4 gene of herpes simplex virus type 1 is dispensable for latency, reactivation and pathogenesis in mice. J Gen Virol, 79: 1603-1611.
          doi: 10.1099/0022-1317-79-7-1603

      11. Lee L F, Wu P, Sui D, et al. 2000. The complete unique long sequence and the overall genomic organization of the GA strain of Marek's disease virus. Proc Natl Acad Sci USA, 97: 6091-6096.
          doi: 10.1073/pnas.97.11.6091

      12. Leibovitz L. 1978. Disease of Poultry. Ames Iowa:Iowa state university Press. 632.

      13. Li H, Liu S, Kong X, et al. 2006. Characterization of the genes encoding UL24, TK and gH proteins from duck enteritis virus (DEV): a proof for the classification of DEV. Virus Genes, 33 (2): 221-227.
          doi: 10.1007/s11262-005-0060-6

      14. Liu F, Ma B, Zhao Y, et al. 2008. Characterization of the gene encoding glycoprotein C of duck enteritis virus. Virus Genes, 37 (3): 328-332.
          doi: 10.1007/s11262-008-0266-5

      15. Mayo M A. 2005. Changes to virus taxonomy 2004. Arch Virol, 150: 189-198.
          doi: 10.1007/s00705-004-0429-1

      16. McGeoch D J, Cunningham C, McIntyre G, et al. 1991. Comparative sequence analysis of the long repeat regions and adjoining parts of the long unique regions in the genomes of herpes simplex viruses types 1 and 2. J Gen Virol, 72: 3057-3075.
          doi: 10.1099/0022-1317-72-12-3057

      17. Pan H, Cao R, Liu L, et al. 2008. Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene. Virus Res, 136 (1-2): 152-156.
          doi: 10.1016/j.virusres.2008.05.003

      18. Pan H. 2007. Cloning and expression of duck enteritis virus gB gene and preliminary application of its product. Mr. Thesis, address: Gansu Agricultural University. 12-14.

      19. Singh J, Wagner E K. 1993. Transcriptional analysis of the herpes simplex virus type 1 region containing the TRL/ UL junction. Virology, 196: 220-231.
          doi: 10.1006/viro.1993.1470

      20. Telford E A, Watson M S, McBride K, et al. 1992. The DNA sequence of equine herpesvirus-1. Virology, 189: 304-316.
          doi: 10.1016/0042-6822(92)90706-U

      21. Vlcek C, Benes V, Lu Z, et al. 1995. Nucleotide sequence analysis of a 30-kb region of the bovine herpesvirus 1 genome which exhibits a colinear gene arrangement with the UL21 to UL4 genes of herpes simplex virus. Virology, 210: 100-108.
          doi: 10.1006/viro.1995.1321

      22. Xie Z X, Xie Z Q, Liu J B. 2000. Detection of duck plague virus using the polymerase chain reaction. Chin J Vet Drug 34: 10-12. (in Chinese)

      23. Yamada H, Jiang Y M, Oshima S, et al. 1998. Characterization of the UL4 gene product of herpes simplex virus type 2. Arch Virol, 143: 1199-1207.
          doi: 10.1007/s007050050367

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      Molecular Characterization of the Duck Enteritis Virus UL4 Gene

        Corresponding author: Rui-bing CAO, crb@njau.edu.cn
      • 1. Key Laboratory of Animal Disease Diagnosis and Immunology, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China
      • 2. Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016, China
      • 3. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
      • Received Date: 16 September 2008
      • Accepted Date: 16 January 2009

      Abstract: Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious and fatal disease. In the present article, the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction (PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame (ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups, and the duck enteritis virus branched separately, closely related to the Mardiviruses group comprising Gallid herpesvirus 2 (GaHV-2), Gallid herpesvirus 3 (GaHV-3) and Meleagrid herpesvirus 1 (MeHV-1). The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.

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