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Volume 24 Issue 3
June 2009
    Citation: Hong TIAN, Jing-yan WU, Shuang-hui YIN, You-jun SHANG, Zi-ping MAN, Na ZHAO, Ye JIN, Xiang-tao LIU. Genetic Variation Analyses of nsp2 Gene of PRRSV in Ningxia Hui Autonomous Region of China .VIROLOGICA SINICA, 2009, 24(3) : 221-226.  http://dx.doi.org/10.1007/s12250-009-3017-4
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    Genetic Variation Analyses of nsp2 Gene of PRRSV in Ningxia Hui Autonomous Region of China

    cstr: 32224.14.s12250-009-3017-4

    • Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    • Corresponding author: Xiang-tao LIU, hnxiangtao@163.com
    • Received Date: 10 December 2008
      Accepted Date: 12 April 2009
      Available online: 01 June 2009
    • To gain a better understanding of the genetic diversity and evolution of PRRSV in the Ningxia Hui Nationality Autonomous Region (Ningxia) of China, the nsp2 genes from a series of PRRSV strains collected from the region in 2007 were partially sequenced. These sequences were then analyzed along with the classical strain (ch-1a) and two other epidemic strains SD (3) and SD2006. Comparison of the nucleotide sequence with ch-1a indicated that nsp2 genes of seventeen Ningxia isolates (NX strain) have deletions of 87 nucleotides. Sequence analysis indicated that homology between the Ningxia strain and ch-1a was 60.3%-79.9% in the nucleotide sequence, and homology between the NX strains and SD strains was 80.3%-98.8% in the nucleotide sequence. The nsp2 genes of the seventeen isolates had 74.9%-100% nucleotide sequence identities with each other. This study was undertaken to assess the regional variation of prevalent PRRSV and to establish a sequence database for PRRSV molecular epidemiological studies.
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      7. Ropp S L, Wees C E, Fang Y, et al. 2004. Characterization of emerging European-like PRRSV isolates in the United States. J Virol, 78: 3684-3703.
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      11. Ropp S L, Wees C E M, Fang Y, et al. 2004. Characteri-zation of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States. J Virol, 78: 3684-3703.
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      12. Shen S, Kwang J, Liu W, et al. 2000. Determination of the complete nucleotide sequence of a vaccine strain of porcine reproductive and respiratory syndrome virus and identification of the Nsp2 gene with a unique insertion. Arch Virol, 145: 871-883.
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      Genetic Variation Analyses of nsp2 Gene of PRRSV in Ningxia Hui Autonomous Region of China

        Corresponding author: Xiang-tao LIU, hnxiangtao@163.com
      • Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
      • Received Date: 10 December 2008
      • Accepted Date: 12 April 2009

      Abstract: To gain a better understanding of the genetic diversity and evolution of PRRSV in the Ningxia Hui Nationality Autonomous Region (Ningxia) of China, the nsp2 genes from a series of PRRSV strains collected from the region in 2007 were partially sequenced. These sequences were then analyzed along with the classical strain (ch-1a) and two other epidemic strains SD (3) and SD2006. Comparison of the nucleotide sequence with ch-1a indicated that nsp2 genes of seventeen Ningxia isolates (NX strain) have deletions of 87 nucleotides. Sequence analysis indicated that homology between the Ningxia strain and ch-1a was 60.3%-79.9% in the nucleotide sequence, and homology between the NX strains and SD strains was 80.3%-98.8% in the nucleotide sequence. The nsp2 genes of the seventeen isolates had 74.9%-100% nucleotide sequence identities with each other. This study was undertaken to assess the regional variation of prevalent PRRSV and to establish a sequence database for PRRSV molecular epidemiological studies.

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