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Volume 24 Issue 6
December 2009
    Citation: Sheng-li MENG, Ge-Lin XU, Jie WU, Xiao-Ming YANG, Jia-Xin YAN. A Comparison of Complete Genome Sequences of a Rabies Virus Chinese Isolate SH06 with the Vaccine Strains .VIROLOGICA SINICA, 2009, 24(6) : 529-536.  http://dx.doi.org/10.1007/s12250-009-3030-7
    shu

    A Comparison of Complete Genome Sequences of a Rabies Virus Chinese Isolate SH06 with the Vaccine Strains

    cstr: 32224.14.s12250-009-3030-7

    • Center of Rabies Diagnosis, Wuhan Institute of Biological Products, Wuhan 430060, China

    • Corresponding author: Jia-Xin YAN, yanjx45@163.com
    • Received Date: 09 February 2009
      Accepted Date: 24 July 2009
      Available online: 01 December 2009

      Fund Project: National High-Tech Research and Development Program of China 2007AA022402

    • In this study, we determined the complete nucleotide and deduced amino acid sequence of a primary isolate of rabies virus (SH06) obtained from the brain of a rabid dog. The overall length of the genome was 11 924 nucleotides. Comparison of the genomic sequence showed the homology of SH06 at nucleotide level with full-length genomes of reference vaccine strains ranged from 82.2% with the PV strain to 86.9% with the CTN strain. A full-length genome-based phylogenetic analysis was performed with sequences available from GenBank. Phylogenetic analysis of the complete genome sequences indicated that the SH06 exhibited the highest homology with rabies street virus BD06 and CTN vaccine strain originated from China.
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      7. Hillis D M, Bull J J. 1993. An Empirical Test of Bootstrapping as a Method for Assessing Confidence in Phylogenetic Analysis. Systematic Biology, 42: 182-192.
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      8. Ito N, Kakemizu M, Ito K A, et al. 2001. A comparison of complete genome sequences of the attenuated RC-HL strain of rabies virus used for production of animal vaccine in Japan, and the parental Nishigahara strain. Microbiol Immunol, 45: 51-58.
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      9. Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through com-parative studies of nucleotide sequences. J Mol Evol, 16: 111-120.
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      10. Meng S L, Yan J X, Xu G L, et al. 2007. A molecular epidemiological study targeting the glycoprotein gene of rabies virus isolates from China. Virus Res, 124: 125-138.
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      11. Metlin A, Paulin L, Suomalainen S, et al. 2008. Characterization of Russian rabies virus vaccine strain RV-97. Virus Res, 132: 242-247.
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      12. WHO. 2005. WHO Expert consultation on Rabies, 2005. First Report; Geneva: WHO technical report series, 931: 1-88.

      13. Meng S L, , Xu G L, Yan J X, et al. 2007, Molecular Epidemiology and Sequencing of the G-L Intergenic Region of Rabies Viruses Isolated in China. Virol Sin, 22: 26-33.
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      14. Tamura K, Dudley J, Nei M, et al. 2007, MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol Biol Evol, 24: 1596-1599.
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      15. Tordo N, Poch O, Ermine A, et al. 1986, Walking along the Rabies Genome: Is the Large G-L Intergenic Region a Remnant Gene? PNAS, 83: 3914-3918.
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          doi: 10.1111/mim.2005.49.issue-8

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      A Comparison of Complete Genome Sequences of a Rabies Virus Chinese Isolate SH06 with the Vaccine Strains

        Corresponding author: Jia-Xin YAN, yanjx45@163.com
      • Center of Rabies Diagnosis, Wuhan Institute of Biological Products, Wuhan 430060, China
      • Received Date: 09 February 2009
      • Accepted Date: 24 July 2009
      Fund Project:  National High-Tech Research and Development Program of China 2007AA022402

      Abstract: In this study, we determined the complete nucleotide and deduced amino acid sequence of a primary isolate of rabies virus (SH06) obtained from the brain of a rabid dog. The overall length of the genome was 11 924 nucleotides. Comparison of the genomic sequence showed the homology of SH06 at nucleotide level with full-length genomes of reference vaccine strains ranged from 82.2% with the PV strain to 86.9% with the CTN strain. A full-length genome-based phylogenetic analysis was performed with sequences available from GenBank. Phylogenetic analysis of the complete genome sequences indicated that the SH06 exhibited the highest homology with rabies street virus BD06 and CTN vaccine strain originated from China.

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