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Volume 26 Issue 4
August 2011
    Citation: Nai-tong Yu, Tuan-cheng Feng, Yu-liang Zhang, Jian-hua Wang, Zhi-xin Liu. Bioinformatic Analysis of BBTV Satellite DNA in Hainan .VIROLOGICA SINICA, 2011, 26(4) : 279-284.  http://dx.doi.org/10.1007/s12250-011-3196-7
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    Bioinformatic Analysis of BBTV Satellite DNA in Hainan

    cstr: 32224.14.s12250-011-3196-7

    • Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101, China

    • Corresponding author: Zhi-xin Liu, itbblzx@yahoo.com.cn
    • Received Date: 09 April 2011
      Accepted Date: 01 July 2011
      Available online: 01 August 2011

      Fund Project: The Central Level, Scientific Research Institutes for Basic R & D Special Fund Business ITBB110303

    • Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiation protein of BBTV DNA1.
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      1. Bell K E, Dale J L, Ha C V, et al. 2002. Characterisation of Rep-encoding compenents associated with banana bunchy top nanovirus in Vietnam. Arch Virol, 147: 695-707.
          doi: 10.1007/s007050200019

      2. Burns T M, Harding R M, Dale J L. 1995. The genome organization of Banana bunchy top virus: analysis of six ssDNA components. J Gen Virol, 76: 1471-1482.
          doi: 10.1099/0022-1317-76-6-1471

      3. Dale J L. 1987. Banana bunchy top: aneconomically important tropical plant virus disease. Adv Virus Res, 33: 301-325.
          doi: 10.1016/S0065-3527(08)60321-8

      4. Fauquet C M, Mayo M A, Maniloff J, et al. 2005. Virus Taxonomy, Classification and Nomenclature of Viruses. In: Eighth Report of the International Committee on the Taxonomy of Viruses. Amsterdam, Boston, Heidelierg: Elsevier Academic Press.

      5. Harding R M, Burns T M, Dale J L. 1991. Virus-like particles associated with banana bunchy top disease contain small single-stranded DNA. J Gen Virol, 72:225-230.
          doi: 10.1099/0022-1317-72-2-225

      6. Harding R M, Burns T M, Hafner G J, et al. 1993. Nucleotide-sequence of one-component of the Banana bunchy top virus genome contains a putative replicase gene. J Gen Virol, 74: 323-328.
          doi: 10.1099/0022-1317-74-3-323

      7. Hermann S R, Becker D K, Harding R M, et al. 2001. Promoters derived from banana bunchy top virus-associated components S1 and S2 drive transgene expression in both tobacco and banana. Plant Cell Rep, 20: 642-646.
          doi: 10.1007/s002990100348

      8. Horser C L, Karan M, Harding R M, et al. 2001. Additional Rep-encoding DNAs associated with banana bunchy top virus. Arch Virol, 146: 71-86.
          doi: 10.1007/s007050170192

      9. Karan M, Harding R M, Dale J L. 1997. Association of banana bunchy top virus DNA components 2 to 6 with banana bunchy disease. Mol Plant Pathol, On-line: 1-16.

      10. Wu R Y, You L R, Soong T S. 1994. Nucleotide sequence of two circular single-stranded DNA's associated with banana bunchy top virus. Phytopathology, 84: 952–958.
          doi: 10.1094/Phyto-84-952

      11. Yu N T, Zhang Y L, Wang J H, et al. 2011. Characteristics analysis of banana bunchy top virus Rep protein and establishment of its purification system. J Agric Sci Technol, 13(2): 31-37.

      12. Zhou Z Z, Lin Q Y, Xie L H, et al. 1993. Studies on banana bunchy top I. Incidence, epidemics and distribution of the disease in Fujian. J Fujian Agric Univ (Nat Sci ed), 22(3): 305-310.

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      Bioinformatic Analysis of BBTV Satellite DNA in Hainan

        Corresponding author: Zhi-xin Liu, itbblzx@yahoo.com.cn
      • Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101, China
      • Received Date: 09 April 2011
      • Accepted Date: 01 July 2011
      Fund Project:  The Central Level, Scientific Research Institutes for Basic R & D Special Fund Business ITBB110303

      Abstract: Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiation protein of BBTV DNA1.

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