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Volume 31 Issue 2
April 2016
    Citation: Chenglin Deng, Siqing Liu, Qiuyan Zhang, Mingyue Xu, Honglei Zhang, Dayong Gu, Lei Shi, Jian'an He, Gengfu Xiao, Bo Zhang. Isolation and characterization of Zika virus imported to China using C6/36 mosquito cells .VIROLOGICA SINICA, 2016, 31(2) : 176-179.  http://dx.doi.org/10.1007/s12250-016-3778-5
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    Isolation and characterization of Zika virus imported to China using C6/36 mosquito cells

    • 1.

      Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • 2.

      University of Chinese Academy of Sciences, Beijing 100049, China

    • 3.

      The Central Laboratory of Health Quarantine, Shenzhen Travel Healthcare Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518033, China

    • 4.

      State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • Corresponding author: Bo Zhang, zhangbo@wh.iov.cn, ORCID: 0000-0002-8895-3679
    • Published Date: 22 April 2016
      Available online: 01 April 2016
    • Here, we describe a cell culture-based procedure for isolating the infectious ZIKV (GenBank KU963796) from a human serum sample (ca. 50 μL) with an extremely low viral load (Ct value = 32).
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      1. Barzon L, Pacenti M, Berto A, et al. 2016. Euro Surveill. 2016. 21: 1-5.

      2. Deng YQ, Zhao H, Li XF, et al. 2016. Sci China Life Sci. Epub ahead of print, 59: 428-430.

      3. Hills SL, Russell K, Hennessey M, et al. 2016. MMWR (Morbidity and mortality weekly report), 65: 215-216.
          doi: 10.15585/mmwr.mm6508e2

      4. Kraemer MUG, Sinka ME, Duda KA, et al. 2015. eLife, 4: e08347.

      5. Gubler DJ, Kuno G, Markoff L. 2007. Fields Virology, 5th Edition, Knipe D and Howley PM eds. Philadelphia : Lippincott Williams & Wilkins, pp. 1154-1227.

      6. Li J, Xiong Y, Wu W, et al. 2016. Emerg Infect Dis, Epub ahead of print. doi: 10.3201/eld2206.160273

      7. Liu S-Q, Zhang B. 2016. Virol Sin, 31: 1-2.
          doi: 10.1007/s12250-016-3746-0

      8. Shinohara K, Kutsuna S, Takasaki T, et al. 2016. J Travel Med, 23. doi: 10.1093/jtm/tav011.

      9. Triunfol M. 2016. Lancet Infect Dis, 16: 156-157.
          doi: 10.1016/S1473-3099(15)00548-4

      10. World Health Organization. 2016. 12 Febuary 2016, http://apps.who.int/iris/bitstream/10665/204371/1/zikasitrep_12Feb2016_eng.pdf.

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      Isolation and characterization of Zika virus imported to China using C6/36 mosquito cells

        Corresponding author: Bo Zhang, zhangbo@wh.iov.cn
      • 1. Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • 2. University of Chinese Academy of Sciences, Beijing 100049, China
      • 3. The Central Laboratory of Health Quarantine, Shenzhen Travel Healthcare Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518033, China
      • 4. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • Published Date: 22 April 2016

      Abstract: Here, we describe a cell culture-based procedure for isolating the infectious ZIKV (GenBank KU963796) from a human serum sample (ca. 50 μL) with an extremely low viral load (Ct value = 32).

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