Sequence Analysis of the 1ef-3 Gene of Helicoverpa armigera Single-nucleocapsid Nucle0pOlyhedr0virus
Abstract: An lef一3 gene of HaSNPV was identified.It was 1ocalized in Bam H 工一F fragment of the genome.Nucleotide sequencing showed the open reading frame was 1 1 40 nt,encoded 379 amino acids with a predicted size of 44kD.A ”TATA”box was found in 40~ 43nt upstream of the transcriptiona1 start codon ATG.A typical poly(A)signal,AATAAA,was found in the downstream of the tran— scriptional stop codon.HaSPV LEF一3 protein also contains the conserved motif。which exists in nearly 20 kinds of SSBs,SO HaSNPV LEF一3 protein iS a probable SSB protein