Citation: SHEN Yang, LIN Jiao-jiao, LI Ming, LIU Jin-ming, CAI You-min, WU Xiang-fu. The Diagnostic Research of Hepatitis C with Recombinant Antigen .VIROLOGICA SINICA, 2005, 20(2) : 206-208.

The Diagnostic Research of Hepatitis C with Recombinant Antigen

  • Available online: 20 April 2005
  • In order to improve the diagnostic efficiency of the hepatitis C in China, the DNA fragments of core, C33c,NS4 in Hepatitis C virus(HCV) were connected and subcloned to pET24a(+). The pET24a-CCN was highly expressed with the inducement IPTG in the E.coli BL21(DE3). In 1L bacterial culture medium,30mg purified recombinant proteins were available using His Binding Resin under denatured condition. Used as diagnostic antigen to detect the human hepatitis C by ELISA, The results showed that the 100% positive coincidence rate (66/66) and 94.5% negative coincidence rate (86/91) were obtained. To detect 46 positive HAV sera and 46 positive HBs sera, all the results were negative. At the same time, the freeze-dried recombinant antigen detects 66 HCV positive sera, all the results were positive. Therefore, the recombinant antigen was sensitivity, specialty and stability to detect hepatitis C. It was an ideal reagent to detect hepatitis C.

  • 加载中
  • 加载中

Article Metrics

Article views(3921) PDF downloads(29) Cited by()

Related
Proportional views

    The Diagnostic Research of Hepatitis C with Recombinant Antigen

    • 1. 

    Abstract: In order to improve the diagnostic efficiency of the hepatitis C in China, the DNA fragments of core, C33c,NS4 in Hepatitis C virus(HCV) were connected and subcloned to pET24a(+). The pET24a-CCN was highly expressed with the inducement IPTG in the E.coli BL21(DE3). In 1L bacterial culture medium,30mg purified recombinant proteins were available using His Binding Resin under denatured condition. Used as diagnostic antigen to detect the human hepatitis C by ELISA, The results showed that the 100% positive coincidence rate (66/66) and 94.5% negative coincidence rate (86/91) were obtained. To detect 46 positive HAV sera and 46 positive HBs sera, all the results were negative. At the same time, the freeze-dried recombinant antigen detects 66 HCV positive sera, all the results were positive. Therefore, the recombinant antigen was sensitivity, specialty and stability to detect hepatitis C. It was an ideal reagent to detect hepatitis C.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return