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Volume 25 Issue 1
February 2010
    Citation: Ze LIU, Wei-dong LI, Ming-bo SUN, Lei MA, Zi-quan GUO, Shu-de JIANG, Guo-yang LIAO, Jing-si YANG, Chang-gui LI. Screening of a High Growth Influenza B Virus Strain in Vero Cells .VIROLOGICA SINICA, 2010, 25(1) : 65-70.  http://dx.doi.org/10.1007/s12250-010-3066-8
    shu

    Screening of a High Growth Influenza B Virus Strain in Vero Cells

    cstr: 32224.14.s12250-010-3066-8
    • 1.

      Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China

    • 2.

      National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China

    • Corresponding author: Jing-si YANG, yjs@imbcams.com.cn
      Chang-gui LI, changguili@yahoo.com.cn
    • Received Date: 28 April 2009
      Accepted Date: 07 September 2009
      Available online: 01 February 2010

      Fund Project: National "863 Project" 2006AA02Z409National health project 200802023Yunnan sciences and technology cooperation 2006XY29

    • Due to the insufficient supply of embryonated chicken eggs, the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemergence of a pandemic influenza virus. The Vero cell is one of the ideal options since it was used for producing many kinds of human vaccines. However, most of the influenza viruses can not grow well in Vero cells. To develop a new influenza vaccine with Vero cells as a substrate, the virus needs to adapt to this cell substrate to maintain high growth characteristics. By serial passages in Vero cells, the B/Yunnan/2/2005va (B) strain was successfully adapted to Vero cells, with the hemagglutination titer (HAT) of the virus reaching 1:512. The high growth characteristic of this strain is stable up to 21 passages. The strain was identified by hemagglutination inhibition (HAI) test and sequencing respectively; the HA1 gene sequence of the virus was cloned and analyzed. The screening and establishment of high growth B virus provides an important tool for influenza vaccine production in Vero cells.
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          doi: 10.1016/j.vaccine.2007.07.027

      3. Chen J M, Guo Y J, Wu K Y, et al. 2001. Two pedigree and the origin of influenza B virus evolution. Chin J Virol, 17 (4): 322-327. (in Chinese)

      4. Govorkova E A, Murti G, Meignier B, et al. 1996. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol, 70 (8): 5519-55241.

      5. Jackson D, Cadman A, Zurcher T, et al. 2002.A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA. J Virol, 76: 11744-117471.
          doi: 10.1128/JVI.76.22.11744-11747.2002

      6. Lu J P, Zhou R, Ou Z Y, et al. 2008. Isolate the Guangdong strain of influenza B virus and analyzed it's HA gene fragments. Guangdong Med Sci, 7 (29): 1120-1122. (in Chinese)

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      8. Qi L, Wu K Y, Li X, et al. 2004. Screen for high yield influenza B virus in MDCK cells and cloned the whole genome. Chin J Virol, 20 (3): 195-199. (in Chinese)

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      10. Simonsen L, Taylor R J, Viboud C, et al. 2007. Mortality benefits of influenza vaccination in elderly people: an ongoing controversy. Lancet Infectious Disease, 7: 658-666.
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      11. Wu S H, Shu Y L. 2006. The application perspective of the influenza vaccine in Vero cells. Chin J Virol, 22 (1): 70-73. (in Chinese)

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      Screening of a High Growth Influenza B Virus Strain in Vero Cells

        Corresponding author: Jing-si YANG, yjs@imbcams.com.cn
        Corresponding author: Chang-gui LI, changguili@yahoo.com.cn
      • 1. Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China
      • 2. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China
      • Received Date: 28 April 2009
      • Accepted Date: 07 September 2009
      Fund Project:  National "863 Project" 2006AA02Z409National health project 200802023Yunnan sciences and technology cooperation 2006XY29

      Abstract: Due to the insufficient supply of embryonated chicken eggs, the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemergence of a pandemic influenza virus. The Vero cell is one of the ideal options since it was used for producing many kinds of human vaccines. However, most of the influenza viruses can not grow well in Vero cells. To develop a new influenza vaccine with Vero cells as a substrate, the virus needs to adapt to this cell substrate to maintain high growth characteristics. By serial passages in Vero cells, the B/Yunnan/2/2005va (B) strain was successfully adapted to Vero cells, with the hemagglutination titer (HAT) of the virus reaching 1:512. The high growth characteristic of this strain is stable up to 21 passages. The strain was identified by hemagglutination inhibition (HAI) test and sequencing respectively; the HA1 gene sequence of the virus was cloned and analyzed. The screening and establishment of high growth B virus provides an important tool for influenza vaccine production in Vero cells.

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