Serving the Society Since 1986
Advanced Search
Volume 25 Issue 2
April 2010
    Citation: Ruo-feng SHANG, Jian-ping LIANG, Zhong-yuan NA, Hong-jun YANG, Yu LU, Lan-ying HUA, Wen-zhu GUO, Ying CUI, Ling WANG. In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV* .VIROLOGICA SINICA, 2010, 25(2) : 145-150.  http://dx.doi.org/10.1007/s12250-010-3082-8
    shu

    In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV*

    cstr: 32224.14.s12250-010-3082-8
    • 1.

      Key Laboratory of New Animal Medicine Project, Chinese Academy of Agricultural Sciences, Lanzhou Insititute of Animal Husbandry & Veterinary Pharamaceutics Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China

    • 2.

      Yunnan Eco-agricultural Research Institute, Kunming 650106, China

    • Corresponding author: Jian-ping LIANG, Liangjp100@sina.com
    • Received Date: 10 June 2009
      Accepted Date: 12 January 2010
      Available online: 01 April 2010

      Fund Project: Key Technologies Research and Development Program 2004BA519A26

    • NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2g/kg/d or 0.1g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.
    • 加载中

      1. Butt K M, Smith G J D, Chen H, et al.2005. Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003. J Clin Microbiol,43 (11): 5760-5767.
          doi: 10.1128/JCM.43.11.5760-5767.2005

      2. Gai J Y. 2003. Test of statistical hypothesis. In: Methods of Experimental Statistics. The first edition. Beijing: The Publishing Company of China Agriculture, p92-96.

      3. Gan M H. 2002. In: Avian Influenza. The second edition. BeiJing: The Publishing Company of China Agriculture, p181-192.

      4. Guo Y, Wang J. 2006. The new progress of study on arteannuin drugs. Chin J Clin Pharmacol Therap, 11(6): 615-618.(in Chinese)

      5. Huang J R, Liu Y M. 2005. The progress of study on chemical composition and pharmaco-activity of Hedyotic diffusa. Chin Formul Prod, (27): 1329-1332.(in Chinese)

      6. Jiang P, Huang L C. 1995. The studies on cumulative and general toxicity of common stonecrop herb. Liaoning J Trad Chin Med, 22(10): 475-479.(in Chinese)

      7. Johnson C D, Kudsk K A, Fukatsu K, et al.2003. Route of nutrition influences generation of antibody-forming cells and initial defense to an active viral infection in the upper respiratory tract. Ann Surg, 237(4): 565-573.

      8. Kadir T, Kyosuke N, Avin K, et al. 1996. Antiviral effect of Sanicula europaea L. leaves extract on influenza virus-infected cells. Biochem Biophys Res Commun, 225:22-26.
          doi: 10.1006/bbrc.1996.1125

      9. Katz J M, Lu X H, Tumpey T M, et al. 2000. Molecular correlates of influenza A H5N1 virus pathogenesis in mice. J Virol, 74(22): 10807-10810.
          doi: 10.1128/JVI.74.22.10807-10810.2000

      10. Kyoko S, Stefan H, Masato H, et al.2004. PB2 amino acid at position 627 affects replicative efficiency, but not cell tropism, of HongKong H5N1 influenza A viruses in mice. Virology, 320: 258-266.
          doi: 10.1016/j.virol.2003.11.030

      11. Li C J, Yu K Z, Tian G B, et al.2005. Evolution of H9N2 influenza viuses from domestic poultry in Mainland China. Virology, 340(1): 70-83.
          doi: 10.1016/j.virol.2005.06.025

      12. Long J X, Wang Q Z.2005. The sequence analysis and all gene cloning of H5N1 subtype Avian Influenza virus (A/duck/Shandong/093/2004). Acta Microbiol Sin, 45(9): 1840-1842.(in Chinese)

      13. Matrosovich M N, Krauss S, Webster R G. 2001. H9N2 influenza A viruses from poultry in Asia have human viruslike receptor specificity. Virology, 281 (2): 156-162.
          doi: 10.1006/viro.2000.0799

      14. Peiris M, Yuen K Y, Leung C W, et al.1999. Human infection with influenza H9N2. Lancet, 354 (9182): 916-917.
          doi: 10.1016/S0140-6736(99)03311-5

      15. Qing F H. 1990. The enhancement properties of Hedyotic diffusa on mice immune functions. Shanghai J Immunol, (16): 32-34. (in Chinese)

      16. Schmidtke M, Schnittler U, Jahn B, et al. 2001. A rapid assay for evaluation of antiviral activity against coxsackie virus B3, influenza virus A, and herpessimples virus type 1. J Virological Methods, 95: 133-143.
          doi: 10.1016/S0166-0934(01)00305-6

      17. Shang R F, Na Z Y, Yang H J, et al.2008.The Effect of NAS preparation on Killing H5N1 and H9N2 subtype AIV in vitro. J Trad Chin Veter Med,6:5-7.(in Chinese)

      18. Wang J M, Yan J P. 2003.The progress of study on pharmacologic action of common stonecrop herb. Acta Chin Med Pharmacol, 31(4): 57-59.(in Chinese)

      19. Wang X h, Cui Y, Liang J P, et al. 2005.The general and chronic toxicity test of NAS preparation. J Trad Chin Veter Med,4:24-25.(in Chinese)

      20. Yin Z, Liu J H.1997. The laboratory diagnostic techniques of viral diseases. In: Animal Virology, Beijing: Science Press, 291-294.

      21. Zheng H Z. 1997. In: The modern study and application of traditional Chinese medicine. Beijing: Academe publishing company, p221-225.

      22. Zhu X L, Wei X B. 2006. The progress of study on anti-virus Chinese herbal medicine. Chin J Trad Veter Sci, (1): 33-38.(in Chinese)

    • 加载中

    Tables(2)

    PDF

    Article Metrics

    Article views(7062) PDF downloads(11) Cited by()

    Related
    Proportional views

      In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV*

        Corresponding author: Jian-ping LIANG, Liangjp100@sina.com
      • 1. Key Laboratory of New Animal Medicine Project, Chinese Academy of Agricultural Sciences, Lanzhou Insititute of Animal Husbandry & Veterinary Pharamaceutics Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
      • 2. Yunnan Eco-agricultural Research Institute, Kunming 650106, China
      • Received Date: 10 June 2009
      • Accepted Date: 12 January 2010
      Fund Project:  Key Technologies Research and Development Program 2004BA519A26

      Abstract: NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2g/kg/d or 0.1g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.

        HTML