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Volume 25 Issue 2
April 2010
    Citation: Zhe Ren, Chuan-hai Zhang, Lian-jun Wang, Yun-xia Cui, Ren-bin Qi, Chong-ren Yang, Ying-jun Zhang, Xiao-yi Wei, Da-xiang Lu, Yi-fei Wang. In vitro Anti-viral Activity of the Total Alkaloids from Tripterygium hypoglaucum against Herpes Simplex Virus Type 1* .VIROLOGICA SINICA, 2010, 25(2) : 107-114.  http://dx.doi.org/10.1007/s12250-010-3092-6
    shu

    In vitro Anti-viral Activity of the Total Alkaloids from Tripterygium hypoglaucum against Herpes Simplex Virus Type 1*

    cstr: 32224.14.s12250-010-3092-6
    • 1.

      Biomedicine Research and Development Center of Jinan University, Guangzhou 510632, China

    • 2.

      Medical College of Jinan University, Guangzhou 510632, China

    • 3.

      National Engineering Research Center of Genetic Medicine, Guangzhou 510632, China

    • 4.

      Department of Chemistry and Biology, West Anhui University, Lu'an 237012, China

    • 5.

      Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China

    • 6.

      South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510000, China

    • Corresponding author: Da-xiang Lu, 
      Yi-fei Wang, twang-yf@163.com
    • Received Date: 30 July 2009
      Accepted Date: 03 December 2009
      Available online: 01 April 2010

      Fund Project: The State Key Laboratory of Phytochemistry and Plant Resources in West China; Chinese Academy of Sciences O807E21211The State Key Laboratory of Phytochemistry and Plant Resources in West China; Chinese Academy of Sciences O807B11211The Joint Funds of National Science Foundation of China U0632010

    • Herpes simplex virus type 1 (HSV-1) is a commonly occurring human pathogen worldwide. There is an urgent need to discover and develop new alternative agents for the management of HSV-1 infection. Tripterygium hypoglaucum (level) Hutch (Celastraceae) is a traditional Chinese medicine plant with many pharmacological activities such as anti-inflammation, anti-tumor and antifertility. The usual medicinal part is the roots which contain about a 1% yield of alkaloids. A crude total alkaloids extract was prepared from the roots of T. hypoglaucum amd its antiviral activity against HSV-1 in Vero cells was evaluated by cytopathic effect (CPE) assay, plaque reduction assay and by RT-PCR analysis. The alkaloids extract presented low cytotoxicity (CC50 = 46.6 μg/mL) and potent CPE inhibition activity, the 50% inhibitory concentration (IC50) was 6.5 μg/mL, noticeably lower than that of Acyclovir (15.4 μg /mL). Plaque formation was significantly reduced by the alkaloids extract at concentrations of 6.25 μg/mL to 12.5 μg/mL, the plaque reduction ratio reached 55% to 75% which was 35% higher than that of Acyclovir at the same concentration. RT-PCR analysis showed that, the transcription of two important delayed early genes UL30 and UL39, and a late gene US6 of HSV-1 genome all were suppressed by the alkaloids extract, the expression inhibiting efficacy compared to the control was 74.6% (UL30), 70.9% (UL39) and 62.6% (US6) respectively at the working concentration of 12.5μg/mL. The above results suggest a potent anti-HSV-1 activity of the alkaloids extract in vitro.
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      In vitro Anti-viral Activity of the Total Alkaloids from Tripterygium hypoglaucum against Herpes Simplex Virus Type 1*

        Corresponding author: Da-xiang Lu,
        Corresponding author: Yi-fei Wang, twang-yf@163.com
      • 1. Biomedicine Research and Development Center of Jinan University, Guangzhou 510632, China
      • 2. Medical College of Jinan University, Guangzhou 510632, China
      • 3. National Engineering Research Center of Genetic Medicine, Guangzhou 510632, China
      • 4. Department of Chemistry and Biology, West Anhui University, Lu'an 237012, China
      • 5. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
      • 6. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510000, China
      • Received Date: 30 July 2009
      • Accepted Date: 03 December 2009
      Fund Project:  The State Key Laboratory of Phytochemistry and Plant Resources in West China; Chinese Academy of Sciences O807E21211The State Key Laboratory of Phytochemistry and Plant Resources in West China; Chinese Academy of Sciences O807B11211The Joint Funds of National Science Foundation of China U0632010

      Abstract: Herpes simplex virus type 1 (HSV-1) is a commonly occurring human pathogen worldwide. There is an urgent need to discover and develop new alternative agents for the management of HSV-1 infection. Tripterygium hypoglaucum (level) Hutch (Celastraceae) is a traditional Chinese medicine plant with many pharmacological activities such as anti-inflammation, anti-tumor and antifertility. The usual medicinal part is the roots which contain about a 1% yield of alkaloids. A crude total alkaloids extract was prepared from the roots of T. hypoglaucum amd its antiviral activity against HSV-1 in Vero cells was evaluated by cytopathic effect (CPE) assay, plaque reduction assay and by RT-PCR analysis. The alkaloids extract presented low cytotoxicity (CC50 = 46.6 μg/mL) and potent CPE inhibition activity, the 50% inhibitory concentration (IC50) was 6.5 μg/mL, noticeably lower than that of Acyclovir (15.4 μg /mL). Plaque formation was significantly reduced by the alkaloids extract at concentrations of 6.25 μg/mL to 12.5 μg/mL, the plaque reduction ratio reached 55% to 75% which was 35% higher than that of Acyclovir at the same concentration. RT-PCR analysis showed that, the transcription of two important delayed early genes UL30 and UL39, and a late gene US6 of HSV-1 genome all were suppressed by the alkaloids extract, the expression inhibiting efficacy compared to the control was 74.6% (UL30), 70.9% (UL39) and 62.6% (US6) respectively at the working concentration of 12.5μg/mL. The above results suggest a potent anti-HSV-1 activity of the alkaloids extract in vitro.

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