Serving the Society Since 1986
Advanced Search
Volume 25 Issue 2
April 2010
    Citation: Li-chun WANG, Song-qing TANG, Yan-mei TANG, Hong-lin ZHAO, Cheng-hong DONG, Ping-fang CUI, Shao-hui MA, Long-ding LIU, Qi-han LI. A Comparison of the Biological Characteristics of EV 71 C 4 Subtypes from Different Epidemic Strains* .VIROLOGICA SINICA, 2010, 25(2) : 98-106.  http://dx.doi.org/10.1007/s12250-010-3102-8
    shu

    A Comparison of the Biological Characteristics of EV 71 C 4 Subtypes from Different Epidemic Strains*

    cstr: 32224.14.s12250-010-3102-8

    • Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China

    • Corresponding author: Qi-han LI, imbcams.lq@gmail.com
    • †Contributing equally to this work.
    • Received Date: 23 September 2009
      Accepted Date: 16 December 2009
      Available online: 01 April 2010

      Fund Project: National Science and Technology Major Project 2008ZX10004-014

    • The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates suggested that they were highly co-related with genetic identity similar to other previously reported EV71 strains in China. Additionally, these strains were identified to display some obvious proliferation dynamics and plaque morphology when propagated in Vero cells. However, a distinctive difference in pathogenic ability in neonatal mice was found. Some differences in cross neutralization test & immunogenic analysis were also found. All these results are related to the biological characterization of circulating EV71 strains in China and aid in the development of an EV71 vaccine in the future.
    • 加载中

      1. Brown B A, Pallansch M A. 1995. Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus. Virus Res, 39: 195-205.
          doi: 10.1016/0168-1702(95)00087-9

      2. Chan K, Goh K, Chong C, et al. 2003. Epidemic hand, foot, and mouth disease caused by human enterovirus 71, Singapore. Emerg Infect Dis, 9: 78-85.
          doi: 10.3201/eid1301.020112

      3. Chang H W. 2009. Progress in Study on Human Enterovirus Type 71. Chin J Biologicals,22(6): 613-617. (in Chinese)

      4. Chua K B, Chua B H, Lee C S, et al. 2007. Genetic diversity of enterovirus 71 isolated from cases of hand, foot and mouth disease in the 1997, 2000 and 2005 outbreaks, Peninsular Malaysia. Malays J Pathol, 29(2): 69-78.

      5. Ding N Z, Wang X M, Sun S W, et al. 2009. Appearance of mosaic enterovirus 71 in the 2008 outbreak of China.Virus Res, 145(1): 157-161.
          doi: 10.1016/j.virusres.2009.06.006

      6. Ho M, Chen E R, Hsu K H, et al. 1999. An epidemic of enterovirus 71 infection in Taiwan. Taiwan Enterovirus Epidemic Working Group. N Engl J Med, 341(13): 929-935.
          doi: 10.1056/NEJM199909233411301

      7. Hsiung G D, Wang J R. 2000. Enterovirus infections with special reference to enterovirus 71. J Microbiol Immunol Infect, 33(1): 1-8.

      8. Huang W J. 2009. Progress in Study on Enterovirus Type 71. Progress in Microbiology and Immunology, 37(2): 54-59.(in Chinese)

      9. Li R Q, Chen L J, Wang Y M, et al. 2009. Genetic characteristics of enterovirus 71 isolated in Beijing, 2006-2008. Chin J Epidemiol, 30(1): 45-49. (in Chinese)

      10. Li Y, Zhan Z Q, Yu G Q. 2009. Progress in Epidemiology of handfoot-and-mouth disease. Med Inform, 22(7): 1388-1390. (in Chinese)

      11. Lin T Y, Chang L Y, Hsia S H, et al. 2002. The 1998 enterovirus 71 outbreak in Taiwan: pathogenesis and management. Clin Infect Dis, 34, Suppl 2: S52-57.

      12. Lu M, Meng G, He Y X, et al. 2009. Pathology of enterovirus 71 infection: an autopsy study of 5 cases. Chin J Pathology, 38(2): 81-85. (in Chinese)

      13. Ma S H, Liu J S, Wang J J, et al. 2009. Genetic Analysis of the VP1 Region of Human Enterovirus 71 Strains Isolated in Fuyang, China, During 2008.Virologica Sinica, 24 (3): 162-170.
          doi: 10.1007/s12250-009-3033-4

      14. Schmidt NJ, Lennett E H, Ho H H. 1974. An apparently new enterovirus isolated from patients with disease of central nervous system. J Infect Dis, 129: 304-309.
          doi: 10.1093/infdis/129.3.304

      15. Wan J F, Zhu L Y, Liu H, et al. 2008. The Epidemiologic Analysis of Hand, Foot, and Mouth Disease (infected by EV 71) in Fuyang in China. Anhui Med J, 29(4): 344-345.(in Chinese)

      16. Wang J R, Tuan Y C, Tsai H P, et al. 2002. Change of major genotype of enterovirus 71 in outbreaks of handfoot-and-mouth disease in Taiwan between 1998 and 2000. J Clin Microbiol, 40: 10-15.
          doi: 10.1128/JCM.40.1.10-15.2002

      17. Wang Y F, Chou C T, Lei H Y, et al. 2004. A Mouse-Adapted Enterovirus 71 Strain Causes Neurological Disease in Mice after Oral Infection.J Virol,78(15): 7916-7924.
          doi: 10.1128/JVI.78.15.7916-7924.2004

      18. WHO.2008. Outbreak news. Enterovirus, China.Wkly Epidemiol Rec, 83 (19): 169-170.

      19. Yu C K, Chen C C, Chen C L, et al.2000. Neutralizing antibody provided protection against enterovirus type 71 lethal challenge in neonatal mice. J Biomed Sci, 7: 523-528.
          doi: 10.1007/BF02253368

      20. Zhang Y, Nan L J, Wu G S, et al. 2009. The Epidemiologic and Virological Analysis of an Outbreak of Hand, Foot, and Mouth Disease in Inner Mongolia in 2007.Chin J Virol, 25(3): 159-165.(in Chinese)

      21. Zhang Y Y, Zhong J. 2008. HFMD and enterovirus 71. J Microbes Infec, 3(2): 97-99.(in Chinese)

    • 加载中

    Figures(5) / Tables(2)

    PDF

    Article Metrics

    Article views(8235) PDF downloads(16) Cited by()

    Related
    Proportional views

      A Comparison of the Biological Characteristics of EV 71 C 4 Subtypes from Different Epidemic Strains*

        Corresponding author: Qi-han LI, imbcams.lq@gmail.com
      • Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China
      • Received Date: 23 September 2009
      • Accepted Date: 16 December 2009
      Fund Project:  National Science and Technology Major Project 2008ZX10004-014

      Abstract: The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates suggested that they were highly co-related with genetic identity similar to other previously reported EV71 strains in China. Additionally, these strains were identified to display some obvious proliferation dynamics and plaque morphology when propagated in Vero cells. However, a distinctive difference in pathogenic ability in neonatal mice was found. Some differences in cross neutralization test & immunogenic analysis were also found. All these results are related to the biological characterization of circulating EV71 strains in China and aid in the development of an EV71 vaccine in the future.

        HTML