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Volume 26 Issue 6
December 2011
    Citation: Chang Liu, Xiao-hong Kong, Wen-tao Qiao, Yun-qi Geng. Bovine Herpesvirus 1 Protein bICP0 Represses the Transcription of bISG15 in Fetal Bovine Lung Cells * .VIROLOGICA SINICA, 2011, 26(6) : 403-408.  http://dx.doi.org/10.1007/s12250-011-3213-x
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    Bovine Herpesvirus 1 Protein bICP0 Represses the Transcription of bISG15 in Fetal Bovine Lung Cells *

    • 1.

      Key Laboratory of Molecular Microbiology and Technology, Ministry of Education; Key Laboratory of Microbial Functional Genomics (Tianjin); Nankai University, Tianjin 300071, China

    • 2.

      School of Medicine, Nankai University, Tianjin 300071, China

    • Corresponding author: Yun-qi Geng, gengyq@nankai.edu.cn
    • Received Date: 21 July 2011
      Accepted Date: 27 October 2011
      Available online: 01 December 2011

      Fund Project: National Natural Science Foundation of China 30970162National Natural Science Foundation of China 81071343

    • The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1’s infection could repress the basal or inducible transcription of bISG15 in FBL cells. It demonstrates that this repression effect depends on BHV-1 viral infection and new protein synthesis. Our previous work showed that bIRF-3 was the key factor in the stimulation of bISG15 in FBL cells, so the effect of BHV-1 viral protein on bIRF-3 activating the promoter of bISG15 was confirmed. The luciferase assay showed the BHV-1 viral protein bICP0 inhibited the activation of bISG15 promoter stimulated by bIRF-3. Taken together, our work suggested that BHV-1 had some molecular mechanism to resist the cellular bISG15’s antiviral functions.
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      Bovine Herpesvirus 1 Protein bICP0 Represses the Transcription of bISG15 in Fetal Bovine Lung Cells *

        Corresponding author: Yun-qi Geng, gengyq@nankai.edu.cn
      • 1. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education; Key Laboratory of Microbial Functional Genomics (Tianjin); Nankai University, Tianjin 300071, China
      • 2. School of Medicine, Nankai University, Tianjin 300071, China
      • Received Date: 21 July 2011
      • Accepted Date: 27 October 2011
      Fund Project:  National Natural Science Foundation of China 30970162National Natural Science Foundation of China 81071343

      Abstract: The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1’s infection could repress the basal or inducible transcription of bISG15 in FBL cells. It demonstrates that this repression effect depends on BHV-1 viral infection and new protein synthesis. Our previous work showed that bIRF-3 was the key factor in the stimulation of bISG15 in FBL cells, so the effect of BHV-1 viral protein on bIRF-3 activating the promoter of bISG15 was confirmed. The luciferase assay showed the BHV-1 viral protein bICP0 inhibited the activation of bISG15 promoter stimulated by bIRF-3. Taken together, our work suggested that BHV-1 had some molecular mechanism to resist the cellular bISG15’s antiviral functions.

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