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Volume 26 Issue 5
October 2011
    Citation: Wei-wei Pan, Jing Long, Jun-ji Xing, Chun-fu Zheng. Molecular Determinants Responsible for the Subcellular Localization of HSV-1 UL4 Protein .VIROLOGICA SINICA, 2011, 26(5) : 347-356.  http://dx.doi.org/10.1007/s12250-011-3217-6
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    Molecular Determinants Responsible for the Subcellular Localization of HSV-1 UL4 Protein

    • Molecular Virology and Viral Immunology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • Corresponding author: Chun-fu Zheng, zhengcf@wh.iov.cn
    • These authors contributed equally to this work
    • Received Date: 04 August 2011
      Accepted Date: 05 September 2011
      Available online: 01 October 2011

      Fund Project: the National Natural Science Foundation of China 30870120the National Natural Science Foundation of China 81000736the National Natural Science Foundation of China 30900059the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences 20071010-141Major State Basic Research Development Program of China 2010CB530105Major State Basic Research Development Program of China 2011CB504802

    • The function of the herpes simplex virus type 1 (HSV-1) UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein (EYFP), the nuclear export signals (NES) of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattern of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 (CRM1)-dependent manner involving RanGTP hydrolysis.
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      Molecular Determinants Responsible for the Subcellular Localization of HSV-1 UL4 Protein

        Corresponding author: Chun-fu Zheng, zhengcf@wh.iov.cn
      • Molecular Virology and Viral Immunology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • Received Date: 04 August 2011
      • Accepted Date: 05 September 2011
      Fund Project:  the National Natural Science Foundation of China 30870120the National Natural Science Foundation of China 81000736the National Natural Science Foundation of China 30900059the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences 20071010-141Major State Basic Research Development Program of China 2010CB530105Major State Basic Research Development Program of China 2011CB504802

      Abstract: The function of the herpes simplex virus type 1 (HSV-1) UL4 protein is still elusive. Our objective is to investigate the subcellular transport mechanism of the UL4 protein. In this study, fluorescence microscopy was employed to investigate the subcellular localization of UL4 and characterize the transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein (EYFP), the nuclear export signals (NES) of UL4 were for the first time mapped to amino acid residues 178 to 186. In addition, the N-terminal 19 amino acids are identified to be required for the granule-like cytoplasmic pattern of UL4. Furthermore, the UL4 protein was demonstrated to be exported to the cytoplasm through the NES in a chromosomal region maintenance 1 (CRM1)-dependent manner involving RanGTP hydrolysis.

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