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Volume 30 Issue 6
December 2015
    Citation: Pengjuan Liu, Ping Li, Wenting Lyu, Xinqiong Li, Song Li, Fan Yang, Jianbo Huang, Zhiwen Xu, Ling Zhu. Epidemiological study and variation analysis of the porcine kobuvirus 3D gene in Sichuan province, China .VIROLOGICA SINICA, 2015, 30(6) : 460-463.  http://dx.doi.org/10.1007/s12250-015-3632-1
    shu

    Epidemiological study and variation analysis of the porcine kobuvirus 3D gene in Sichuan province, China

    cstr: 32224.14.s12250-015-3632-1

    • Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611134, China

    • Corresponding author: Ling Zhu, abtczl72@126.com
    • Fan Yang, ORCID: 0000-0002-0355-3044
    • Published Date: 30 November 2015
      Available online: 01 December 2015
    • As a viral RNA-dependent polymerase, porcine kobuvirus 3D protein is involved in regulating viral RNA replication and plays an important role in virus proliferation (Verma et al., 2013). Therefore, we first conducted molecular and phylogenic analysis of the porcine kobuvirus 3D region. According to the phylogenic tree, the porcine kobuvirus 3D gene was relatively conserved, and the virus has not evolved dramatically since it was first isolated in 2007. A report by Chen showed that the nucleotide and amino acid identities of the porcine kobuvirus VP1 region range from 97.5%–98.8% and 98.2%–98.7%, respectively (Chen et al., 2013); this variability was less than that observed in our current study. Moreover, some point mutations were observed in the analyzed sequences; these mutations may have changed the conformation and function of the 3D protein. These current findings provide insights into the epidemiology and genetic characteristics of this newly emerging virus.
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      Epidemiological study and variation analysis of the porcine kobuvirus 3D gene in Sichuan province, China

        Corresponding author: Ling Zhu, abtczl72@126.com
      • Fan Yang, ORCID: 0000-0002-0355-3044
      • Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611134, China
      • Published Date: 30 November 2015

      Abstract: As a viral RNA-dependent polymerase, porcine kobuvirus 3D protein is involved in regulating viral RNA replication and plays an important role in virus proliferation (Verma et al., 2013). Therefore, we first conducted molecular and phylogenic analysis of the porcine kobuvirus 3D region. According to the phylogenic tree, the porcine kobuvirus 3D gene was relatively conserved, and the virus has not evolved dramatically since it was first isolated in 2007. A report by Chen showed that the nucleotide and amino acid identities of the porcine kobuvirus VP1 region range from 97.5%–98.8% and 98.2%–98.7%, respectively (Chen et al., 2013); this variability was less than that observed in our current study. Moreover, some point mutations were observed in the analyzed sequences; these mutations may have changed the conformation and function of the 3D protein. These current findings provide insights into the epidemiology and genetic characteristics of this newly emerging virus.

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