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Volume 31 Issue 2
April 2016
    Citation: Xinxin Chen, Jifei Yang, Yanhong Ji, Edward Okoth, Bin Liu, Xiaoyang Li, Hong Yin, Qiyun Zhu. Recombinant Newcastle disease virus expressing African swine fever virus protein 72 is safe and immunogenic in mice .VIROLOGICA SINICA, 2016, 31(2) : 150-159.  http://dx.doi.org/10.1007/s12250-015-3692-2
    shu

    Recombinant Newcastle disease virus expressing African swine fever virus protein 72 is safe and immunogenic in mice

    • 1.

      State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    • 2.

      Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China

    • 3.

      International Livestock Research Institute, Nairobi 00100, Kenya

    • Corresponding author: Hong Yin, yinhong@caas.cn
      Qiyun Zhu, zhuqiyun@caas.cn
    • These authors contributed equally to this work.
    • Received Date: 28 November 2015
      Accepted Date: 25 January 2016
      Published Date: 10 March 2016
      Available online: 01 April 2016
    • African swine fever (ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus (ASFV). Since the first case was described in Kenya in 1921, the disease has spread to many other countries. No commercial vaccines are available to prevent ASF. In this study, we generated a recombinant Newcastle disease virus (rNDV) expressing ASFV protein 72 (p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a mouse model. The recombinant virus, rNDV/p72, replicated well in embryonated chicken eggs and was safe to use in chicks and mice. The p72 gene in rNDV/p72 was stably maintained through ten passages. Mice immunized with rNDV/p72 developed high titers of ASFV p72 specific IgG antibody, and had higher levels of IgG1 than IgG2a. Immunization also elicited T-cell proliferation and secretion of IFN-γ and IL-4. Taken together, these results indicate that rNDV expressing ASFV p72 might be a potential vaccine candidate for preventing ASF.
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      Recombinant Newcastle disease virus expressing African swine fever virus protein 72 is safe and immunogenic in mice

        Corresponding author: Hong Yin, yinhong@caas.cn
        Corresponding author: Qiyun Zhu, zhuqiyun@caas.cn
      • 1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
      • 2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
      • 3. International Livestock Research Institute, Nairobi 00100, Kenya
      • Received Date: 28 November 2015
      • Accepted Date: 25 January 2016
      • Published Date: 10 March 2016

      Abstract: African swine fever (ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus (ASFV). Since the first case was described in Kenya in 1921, the disease has spread to many other countries. No commercial vaccines are available to prevent ASF. In this study, we generated a recombinant Newcastle disease virus (rNDV) expressing ASFV protein 72 (p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a mouse model. The recombinant virus, rNDV/p72, replicated well in embryonated chicken eggs and was safe to use in chicks and mice. The p72 gene in rNDV/p72 was stably maintained through ten passages. Mice immunized with rNDV/p72 developed high titers of ASFV p72 specific IgG antibody, and had higher levels of IgG1 than IgG2a. Immunization also elicited T-cell proliferation and secretion of IFN-γ and IL-4. Taken together, these results indicate that rNDV expressing ASFV p72 might be a potential vaccine candidate for preventing ASF.

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