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Volume 35 Issue 5
October 2020
    Citation: Zhihang Zheng, Min Li, Zhihua Liu, Xia Jin, Jin Sun. Establishment of Murine Infection Models with Biological Clones of Dengue Viruses Derived from a Single Clinical Viral Isolate .VIROLOGICA SINICA, 2020, 35(5) : 626-636.  http://dx.doi.org/10.1007/s12250-020-00229-y
    shu

    Establishment of Murine Infection Models with Biological Clones of Dengue Viruses Derived from a Single Clinical Viral Isolate

    cstr: 32224.14.s12250-020-00229-y
    • 1.

      CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 200031, Shanghai, China

    • 2.

      Chinese Academy of Sciences, University of Chinese Academy of Sciences, 100049, Beijing, China

    • 3.

      Shanghai Public Health Clinical Center, Fudan University, 201508, Shanghai, China

    • Corresponding author: Xia Jin, jinxia@shphc.org.cn, ORCID: 0000-0002-4185-1966
      Jin Sun, jsun01@ips.ac.cn, ORCID: 0000-0002-4181-9317
    • Received Date: 09 November 2019
      Accepted Date: 11 March 2020
      Published Date: 25 May 2020
      Available online: 01 October 2020
    • Dengue virus (DENV) is a single-stranded RNA virus transmitted by mosquitoes in tropical and subtropical regions. It causes dengue fever, dengue hemorrhagic fever and dengue shock syndrome in patients. Each year, 390 million people are estimated to be infected by four serotypes of dengue virus, creating a great burden on global public health and local economy. So far, no antiviral drug is available for dengue disease, and the newly licensed vaccine is far from satisfactory. One large obstacle for dengue vaccine and drug development is the lack of suitable small animal models. Although some DENV infection models have been developed, only a small number of viral strains can infect immunodeficient mice. In this study, with biologically cloned viruses from a single clinical isolate, we have established two mouse models of DENV infection, one is severe lethal infection in immunocompromised mice, and the other resembles self-limited disease manifestations in Balb/c mice with transient blockage of type Ⅰ IFN responses. This study not only offers new small animal models of dengue viral infection, but also provides new viral variants for further investigations on dengue viral pathogenesis.
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      1. Aguirre S, Maestre AM, Pagni S, Patel JR, Savage T, Gutman D, Maringer K, Bernal-Rubio D, Shabman RS, Simon V, Rodriguez-Madoz JR, Mulder LC, Barber GN, Fernandez-Sesma A (2012) DENV inhibits type Ⅰ IFN production in infected cells by cleaving human STING. PLoS Pathog 8:e1002934
          doi: 10.1371/journal.ppat.1002934

      2. Akkina R, Berges BK, Palmer BE, Remling L, Neff CP, Kuruvilla J, Connick E, Folkvord J, Gagliardi K, Kassu A, Akkina SR (2011) Humanized Rag1-/- gammac-/- mice support multilineage hematopoiesis and are susceptible to HIV-1 infection via systemic and vaginal routes. PLoS ONE 6:e20169
          doi: 10.1371/journal.pone.0020169

      3. Ashour J, Morrison J, Laurent-Rolle M, Belicha-Villanueva A, Plumlee CR, Bernal-Rubio D, Williams KL, Harris E, Fernandez-Sesma A, Schindler C, Garcia-Sastre A (2010) Mouse STAT2 restricts early dengue virus replication. Cell Host Microbe 8:410-421
          doi: 10.1016/j.chom.2010.10.007

      4. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI (2013) The global distribution and burden of dengue. Nature 496:504-507
          doi: 10.1038/nature12060

      5. Chen HC, Hofman FM, Kung JT, Lin YD, Wu-Hsieh BA (2007) Both virus and tumor necrosis factor alpha are critical for endothelium damage in a mouse model of dengue virus-induced hemorrhage. J Virol 81:5518-5526
          doi: 10.1128/JVI.02575-06

      6. Crouse J, Kalinke U, Oxenius A (2015) Regulation of antiviral T cell responses by type Ⅰ interferons. Nat Rev Immunol 15:231-242
          doi: 10.1038/nri3806

      7. Green AM, Beatty PR, Hadjilaou A, Harris E (2014) Innate immunity to dengue virus infection and subversion of antiviral responses. J Mol Biol 426:1148-1160
          doi: 10.1016/j.jmb.2013.11.023

      8. Guzman MG, Halstead SB, Artsob H, Buchy P, Farrar J, Gubler DJ, Hunsperger E, Kroeger A, Margolis HS, Martinez E, Nathan MB, Pelegrino JL, Simmons C, Yoksan S, Peeling RW (2010) Dengue: a continuing global threat. Nat Rev Microbiol 8:S7-16
          doi: 10.1038/nrmicro2460

      9. Hotta S (1952) Experimental studies on dengue. I. Isolation, identification and modification of the virus. J Infect Dis 90:1-9
          doi: 10.1093/infdis/90.1.1

      10. Jin X, Lee M, Shu J (2015) Dengue fever in China: an emerging problem demands attention. Emerg Microbes Infect 4:e3
          doi: 10.1038/emi.2015.3

      11. Kato F, Tajima S, Nakayama E, Kawai Y, Taniguchi S, Shibasaki K, Taira M, Maeki T, Lim CK, Takasaki T, Saijo M (2017) Characterization of large and small-plaque variants in the Zika virus clinical isolate ZIKV/Hu/S36/Chiba/2016. Sci Rep 7:16160
          doi: 10.1038/s41598-017-16475-2

      12. Li G, Pan P, He Q, Kong X, Wu K, Zhang W, Liu Y, Huang H, Liu J, Zhang Z, Wu Lai X, Liu X, Wu J (2017) Molecular epidemiology demonstrates that imported and local strains circulated during the 2014 dengue outbreak in Guangzhou, China. Virol Sin 32:63-72
          doi: 10.1007/s12250-016-3872-8

      13. Liang H, Yang R, Liu Z, Li M, Liu H, Jin X (2018) Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice. PLoS ONE 13:e0194860
          doi: 10.1371/journal.pone.0194860

      14. Moser LA, Boylan BT, Moreira FR, Myers LJ, Svenson EL, Fedorova NB, Pickett BE, Bernard KA (2018) Growth and adaptation of Zika virus in mammalian and mosquito cells. PLoS Negl Trop Dis 12:e0006880
          doi: 10.1371/journal.pntd.0006880

      15. Orozco S, Schmid MA, Parameswaran P, Lachica R, Henn MR, Beatty R, Harris E (2012) Characterization of a model of lethal dengue virus 2 infection in C57BL/6 mice deficient in the alpha/beta interferon receptor. J Gen Virol 93:2152-2157
          doi: 10.1099/vir.0.045088-0

      16. Perry ST, Buck MD, Lada SM, Schindler C, Shresta S (2011) STAT2 mediates innate immunity to Dengue virus in the absence of STAT1 via the type Ⅰ interferon receptor. PLoS Pathog 7:e1001297
          doi: 10.1371/journal.ppat.1001297

      17. Plummer EM, Shresta S (2014) Mouse models for dengue vaccines and antivirals. J Immunol Methods 410:34-38
          doi: 10.1016/j.jim.2014.01.001

      18. Prestwood TR, Morar MM, Zellweger RM, Miller R, May MM, Yauch LE, Lada SM, Shresta S (2012) Gamma interferon (IFN-gamma) receptor restricts systemic dengue virus replication and prevents paralysis in IFN-alpha/beta receptor-deficient mice. J Virol 86:12561-12570
          doi: 10.1128/JVI.06743-11

      19. Sabin AB, Schlesinger RW (1945) Production of immunity to dengue with virus modified by propagation in mice. Science 101:640-642
          doi: 10.1126/science.101.2634.640

      20. Sarathy VV, Milligan GN, Bourne N, Barrett AD (2015) Mouse models of dengue virus infection for vaccine testing. Vaccine 33:7051-7060
          doi: 10.1016/j.vaccine.2015.09.112

      21. Shresta S, Sharar KL, Prigozhin DM, Beatty PR, Harris E (2006) Murine model for dengue virus-induced lethal disease with increased vascular permeability. J Virol 80:10208-10217
          doi: 10.1128/JVI.00062-06

      22. St John AL, Rathore APS (2019) Adaptive immune responses to primary and secondary dengue virus infections. Nat Rev Immunol 19:218-230
          doi: 10.1038/s41577-019-0123-x

      23. Stabell AC, Meyerson NR, Gullberg RC, Gilchrist AR, Webb KJ, Old WM, Perera R, Sawyer SL (2018) Dengue viruses cleave STING in humans but not in nonhuman primates, their presumed natural reservoir. Elife 7:e31919
          doi: 10.7554/eLife.31919

      24. Sun J, Li M, Wang Y, Hao P, Jin X (2017) Elaboration of tetravalent antibody responses against dengue viruses using a subunit vaccine comprised of a single consensus dengue envelope sequence. Vaccine 35:6308-6320
          doi: 10.1016/j.vaccine.2017.09.063

      25. Tan GK, Ng JK, Lim AH, Yeo KP, Angeli V, Alonso S (2011) Subcutaneous infection with non-mouse adapted Dengue virus D2Y98P strain induces systemic vascular leakage in AG129 mice. Ann Acad Med Singapore 40:523-532
          doi: 10.2298/VSP1112079P

      26. WHO (2009) In: Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control, New edn. WHO Guidelines Approved by the Guidelines Review Committee, Geneva

      27. Yu CY, Chang TH, Liang JJ, Chiang RL, Lee YL, Liao CL, Lin YL (2012) Dengue virus targets the adaptor protein MITA to subvert host innate immunity. PLoS Pathog 8:e1002780
          doi: 10.1371/journal.ppat.1002780

      28. Zhao H, Zhang FC, Zhu Q, Wang J, Hong WX, Zhao LZ, Deng YQ, Qiu S, Zhang Y, Cai WP, Cao WC, Qin CF (2016) Epidemiological and Virological Characterizations of the 2014 Dengue Outbreak in Guangzhou. China. PLoS ONE 11:e0156548
          doi: 10.1371/journal.pone.0156548

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      Establishment of Murine Infection Models with Biological Clones of Dengue Viruses Derived from a Single Clinical Viral Isolate

        Corresponding author: Xia Jin, jinxia@shphc.org.cn
        Corresponding author: Jin Sun, jsun01@ips.ac.cn
      • 1. CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 200031, Shanghai, China
      • 2. Chinese Academy of Sciences, University of Chinese Academy of Sciences, 100049, Beijing, China
      • 3. Shanghai Public Health Clinical Center, Fudan University, 201508, Shanghai, China
      • Received Date: 09 November 2019
      • Accepted Date: 11 March 2020
      • Published Date: 25 May 2020

      Abstract: Dengue virus (DENV) is a single-stranded RNA virus transmitted by mosquitoes in tropical and subtropical regions. It causes dengue fever, dengue hemorrhagic fever and dengue shock syndrome in patients. Each year, 390 million people are estimated to be infected by four serotypes of dengue virus, creating a great burden on global public health and local economy. So far, no antiviral drug is available for dengue disease, and the newly licensed vaccine is far from satisfactory. One large obstacle for dengue vaccine and drug development is the lack of suitable small animal models. Although some DENV infection models have been developed, only a small number of viral strains can infect immunodeficient mice. In this study, with biologically cloned viruses from a single clinical isolate, we have established two mouse models of DENV infection, one is severe lethal infection in immunocompromised mice, and the other resembles self-limited disease manifestations in Balb/c mice with transient blockage of type Ⅰ IFN responses. This study not only offers new small animal models of dengue viral infection, but also provides new viral variants for further investigations on dengue viral pathogenesis.

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