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Citation: Na Li, Zhen Wang, Rui Wang, Zhe-Rui Zhang, Ya-Nan Zhang, Cheng-Lin Deng, Bo Zhang, Lu-Qing Shang, Han-Qing Ye. In Vitro Inhibition of Alphaviruses by Lycorine [J].VIROLOGICA SINICA, 2021, 36(6) : 1465-1474.  http://dx.doi.org/10.1007/s12250-021-00438-z

In Vitro Inhibition of Alphaviruses by Lycorine

  • Corresponding author: Bo Zhang, zhangbo@wh.iov.cn, ORCID: 0000-0002-8895-3679
    Lu-Qing Shang, shanglq@nankai.edu.cn, ORCID: 0000-0003-4424-3162
    Han-Qing Ye, yehq@wh.iov.cn, ORCID: 0000-0001-7770-4071
  • Received Date: 08 May 2021
    Accepted Date: 08 June 2021
    Published Date: 10 August 2021
    Available online: 01 December 2021
  • Chikungunya virus (CHIKV) is a mosquito-borne alphavirus. As an emerging virus, CHIKV imposes a threat to public health. Currently, there are no vaccines or antivirals available for the prevention of CHIKV infection. Lycorine, an alkaloid from Amaryllidaceae plants, has antiviral activity against a number of viruses such as coronavirus, flavivirus and enterovirus. In this study, we found that lycorine could inhibit CHIKV in cell culture at a concentration of 10 μmol/L without apparent cytotoxicity. In addition, it exhibited broad-spectrum anti-alphavirus activity, including Sindbis virus (SINV), Semliki Forest virus (SFV), and Venezuelan equine encephalomyelitis virus (VEEV). The time of addition studies indicated that lycorine functions at an early post-entry stage of CHIKV life cycle. The results based on two different CHIKV replicons provided further evidence that lycorine exerts its antiviral activity mainly by inhibiting CHIKV translation. Overall, our study extends the antiviral spectrum of lycorine.


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    In Vitro Inhibition of Alphaviruses by Lycorine

      Corresponding author: Bo Zhang, zhangbo@wh.iov.cn
      Corresponding author: Lu-Qing Shang, shanglq@nankai.edu.cn
      Corresponding author: Han-Qing Ye, yehq@wh.iov.cn
    • 1. Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
    • 2. College of Pharmacy and Drug Discovery Center for Infectious Diseases, Nankai University, Tianjin 300350, China

    Abstract: 

    Chikungunya virus (CHIKV) is a mosquito-borne alphavirus. As an emerging virus, CHIKV imposes a threat to public health. Currently, there are no vaccines or antivirals available for the prevention of CHIKV infection. Lycorine, an alkaloid from Amaryllidaceae plants, has antiviral activity against a number of viruses such as coronavirus, flavivirus and enterovirus. In this study, we found that lycorine could inhibit CHIKV in cell culture at a concentration of 10 μmol/L without apparent cytotoxicity. In addition, it exhibited broad-spectrum anti-alphavirus activity, including Sindbis virus (SINV), Semliki Forest virus (SFV), and Venezuelan equine encephalomyelitis virus (VEEV). The time of addition studies indicated that lycorine functions at an early post-entry stage of CHIKV life cycle. The results based on two different CHIKV replicons provided further evidence that lycorine exerts its antiviral activity mainly by inhibiting CHIKV translation. Overall, our study extends the antiviral spectrum of lycorine.