Citation: Yuda Chen, Changcheng Wu, Ruhan A, Li Zhao, Zhongxian Zhang, Wenjie Tan. Perspective on the application of genome sequencing for monkeypox virus surveillance .VIROLOGICA SINICA, 2023, 38(2) : 327-333.  http://dx.doi.org/10.1016/j.virs.2023.03.006

Perspective on the application of genome sequencing for monkeypox virus surveillance

  • Corresponding author: Wenjie Tan, tanwj@ivdc.chinacdc.cn
  • Received Date: 27 September 2022
    Accepted Date: 21 March 2023
    Available online: 25 March 2023
  • Highlights
    1. Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity.
    2. The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained.
    3. Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated.
    4. Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.

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    Perspective on the application of genome sequencing for monkeypox virus surveillance

      Corresponding author: Wenjie Tan, tanwj@ivdc.chinacdc.cn
    • a. School of Public Health, Baotou Medical College, Baotou, 014040, China;
    • b. NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China

    Abstract: Highlights
    1. Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity.
    2. The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained.
    3. Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated.
    4. Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.

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