Citation: Hou-Li Cai, Yao-Wei Huang. Reverse genetics systems for SARS-CoV-2: Development and applications .VIROLOGICA SINICA, 2023, 38(6) : 837-850.

Reverse genetics systems for SARS-CoV-2: Development and applications

  • Corresponding author: Yao-Wei Huang,
  • Received Date: 09 July 2023
    Accepted Date: 07 October 2023
    Available online: 11 October 2023
  • The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused serious harm to human health and struck a blow to global economic development. Research on SARS-CoV-2 has greatly benefited from the use of reverse genetics systems, which have been established to artificially manipulate the viral genome, generating recombinant and reporter infectious viruses or biosafety level 2 (BSL-2)-adapted non-infectious replicons with desired modifications. These tools have been instrumental in studying the molecular biological characteristics of the virus, investigating antiviral therapeutics, and facilitating the development of attenuated vaccine candidates. Here, we review the construction strategies, development, and applications of reverse genetics systems for SARS-CoV-2, which may be applied to other CoVs as well.

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    Reverse genetics systems for SARS-CoV-2: Development and applications

      Corresponding author: Yao-Wei Huang,
    • a. Department of Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China;
    • b. State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, 510642, China;
    • c. Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China

    Abstract: The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused serious harm to human health and struck a blow to global economic development. Research on SARS-CoV-2 has greatly benefited from the use of reverse genetics systems, which have been established to artificially manipulate the viral genome, generating recombinant and reporter infectious viruses or biosafety level 2 (BSL-2)-adapted non-infectious replicons with desired modifications. These tools have been instrumental in studying the molecular biological characteristics of the virus, investigating antiviral therapeutics, and facilitating the development of attenuated vaccine candidates. Here, we review the construction strategies, development, and applications of reverse genetics systems for SARS-CoV-2, which may be applied to other CoVs as well.

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