Citation: Yabin Hu, Jun Chen, Jing Yang, Zhanpeng Liu, Xiaoyue Zhang, Qian Wu, Lulu Liu, Shishan Teng, Rongzhang He, Bo Liu, Xingyu Zheng, Rui Lu, Dong Pan, You Wang, Liting Peng, Hongying chen, Yi-Ping Li, Wenpei Liu, Xiaowang Qu. Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses .VIROLOGICA SINICA, 2024, 39(1) : 169-172.  http://dx.doi.org/10.1016/j.virs.2023.10.008

Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses

  • Highlights
    1. Five broad-spectrum mAbs identified from COVID-19 convalescents and vaccinees possessed the ability to recognize spike S2 of pan-coronaviruses.
    2. Five mAbs targeted distinct but conserved epitopes on spike S2 of pan-coronaviruses.
    3. Three of these five mAbs were competitively bound to the fusion peptide epitopes of coronavirus spike protein.

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  • 10.1016j.virs.2023.10.008-ESM.docx
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    Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses

      Corresponding author: Hongying chen, chenhy@nwsuaf.edu.cn
      Corresponding author: Yi-Ping Li, lyiping@mail.sysu.edu.cn
      Corresponding author: Wenpei Liu, wenpeiliu_2008@foxmail.com
      Corresponding author: Xiaowang Qu, quxiaowang@163.com
    • a. College of Basic Medical Sciences, Hengyang Medical School, University of South China & MOE Key Lab of Rare Pediatric Diseases, Hengyang, 421001, China;
    • b. Translational Medicine Institute, The First People's Hospital of Chenzhou, Hengyang Medical School, University of South China, Chenzhou, 423000, China;
    • c. College of Life Sciences, Northwest A&F University, Yangling, 712100, China;
    • d. Institute of Human Virology, Department of Pathogen Biology and Biosecurity, and Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China;
    • e. School of Public Health, University of South China, Hengyang, 421001, China

    Abstract: Highlights
    1. Five broad-spectrum mAbs identified from COVID-19 convalescents and vaccinees possessed the ability to recognize spike S2 of pan-coronaviruses.
    2. Five mAbs targeted distinct but conserved epitopes on spike S2 of pan-coronaviruses.
    3. Three of these five mAbs were competitively bound to the fusion peptide epitopes of coronavirus spike protein.

    Reference (15) Relative (20)

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