Citation: Xincheng Ni, Kai Wang, Yinze Han, Jian Lei. Structural analysis of conformational changes in the mpox virus A7 protein .VIROLOGICA SINICA, 2024, 39(2) : 331-334.  http://dx.doi.org/10.1016/j.virs.2023.12.006

Structural analysis of conformational changes in the mpox virus A7 protein

  • Corresponding author: Jian Lei, leijian@scu.edu.cn
  • Received Date: 14 September 2023
    Accepted Date: 22 December 2023
    Available online: 28 December 2023
  • Highlights
    1. Phospholipid-binding abilities of mpox virus A7 protein and its truncations are investigated.
    2. The structures of the N-terminal truncations of A7 protein (A7N121 and A7N137) are determined.
    3. Conformational changes of the conserved linking helix in A7 are illustrated.
    4. A structural model of the full-length A7 protein is proposed.

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  • 10.1016j.virs.2023.12.006-ESM.docx
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    2. Condit, R.C., Moussatche, N., Traktman, P., 2006. In a nutshell:structure and assembly of the vaccinia virion. Adv. Virus Res. 66, 31-124.

    3. Isidro, J., Borges, V., Pinto, M., Sobral, D., Santos, J.D., Nunes, A., Mixão, V., Ferreira, R., Santos, D., Duarte, S., Vieira, L., Borrego, M.J., Núncio, S., de Carvalho, I.L., Pelerito, A., Cordeiro, R., Gomes, J.P., 2022. Phylogenomic characterization and signs of microevolution in the 2022 multi-country outbreak of monkeypox virus. Nat. Med. 28,1569-1572.

    4. Kolli, S., Meng, X., Wu, X., Shengjuler, D., Cameron, C.E., Xiang, Y., Deng, J., 2015. Structure-function analysis of vaccinia virus H7 protein reveals a novel phosphoinositide binding fold essential for poxvirus replication. J. Virol. 89, 2209-2219.

    5. Krijnse Locker, J., Chlanda, P., Sachsenheimer, T., Brügger, B., 2013. Poxvirus membrane biogenesis:rupture not disruption. Cell Microbiol. 15, 190-199.

    6. Kugelman, J.R., Johnston, S.C., Mulembakani, P.M., Kisalu, N., Lee, M.S., Koroleva, G., McCarthy, S.E., Gestole, M.C., Wolfe, N.D., Fair, J.N., Schneider, B.S., Wright, L.L., Huggins, J., Whitehouse, C.A., Wemakoy, E.O., Muyembe-Tamfum, J.J., Hensley, L.E., Palacios, G.F., Rimoin, A.W., 2014. Genomic variability of monkeypox virus among humans, Democratic Republic of the Congo. Emerg. Infect. Dis. 20, 232-239.

    7. Luo, B.H., Springer, T.A., 2006. Integrin structures and conformational signaling. Curr. Opin. Cell Biol. 18, 579-86.

    8. Maruri-Avidal, L., Weisberg, A.S., Moss, B., 2013. Association of the vaccinia virus A11 protein with the endoplasmic reticulum and crescent precursors of immature virions. J. Virol. 87, 10195-10206.

    9. Meng, X., Embry, A., Sochia, D., Xiang, Y., 2007. Vaccinia virus A6L encodes a virion core protein required for formation of mature virion. J. Virol. 81, 1433-1443.

    10. Meng, X., Embry, A., Rose, L., Yan, B., Xu, C., Xiang, Y., 2012. Vaccinia virus A6 is essential for virion membrane biogenesis and localization of virion membrane proteins to sites of virion assembly. J. Virol. 86, 5603-5613.

    11. Meng, X., Rose, L., Han, Y., Deng, J., Xiang, Y., 2017. Vaccinia Virus A6 Is a Two-Domain Protein Requiring a Cognate N-Terminal Domain for Full Viral Membrane Assembly Activity. J. Virol. 91, e02405-16.

    12. Moss, B., 2015. Poxvirus membrane biogenesis. Virology 479-480, 619-626.

    13. Pathak, P.K., Peng, S., Meng, X., Han, Y., Zhang, B., Zhang, F., Xiang, Y., Deng, J., 2018. Structure of a lipid-bound viral membrane assembly protein reveals a modality for enclosing the lipid bilayer. Proc. Natl. Acad. Sci. U.S.A. 115, 7028-7032.

    14. Weisberg, A.S., Maruri-Avidal, L., Bisht, H., Hansen, B.T., Schwartz, C.L., Fischer, E.R., Meng, X., Xiang, Y., Moss, B., 2017. Enigmatic origin of the poxvirus membrane from the endoplasmic reticulum shown by 3D imaging of vaccinia virus assembly mutants. Proc. Natl. Acad. Sci. U.S.A. 114, E11001-E11009.

    15. Wu, X., Meng, X., Yan, B., Rose, L., Deng, J., Xiang, Y., 2012. Vaccinia virus virion membrane biogenesis protein A11 associates with viral membranes in a manner that requires the expression of another membrane biogenesis protein, A6. J. Virol. 86, 11276-11286.

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    Structural analysis of conformational changes in the mpox virus A7 protein

      Corresponding author: Jian Lei, leijian@scu.edu.cn
    • National Clinical Research Center for Geriatrics, and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, China

    Abstract: Highlights
    1. Phospholipid-binding abilities of mpox virus A7 protein and its truncations are investigated.
    2. The structures of the N-terminal truncations of A7 protein (A7N121 and A7N137) are determined.
    3. Conformational changes of the conserved linking helix in A7 are illustrated.
    4. A structural model of the full-length A7 protein is proposed.

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